Locating a problem: Difference between revisions
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== Switch componentsΒ == | == Switch componentsΒ == | ||
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Revision as of 19:05, 7 February 2016
Locating a problem |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Switch components
Problem moves with | Problem situated at |
---|---|
POS | POS |
POS connector | POS connector |
not at all | Chamber assembly or Main unit |
Remove components
The approach described above reached its limitations when more then one component is damaged. In this case some information can be gained by sequentially removing components. This approach can also be used to confirm a suspected defect in the main unit. When removing components from the running instrument it is of special importance to observe the ESD guidelines, see MiPNet14.01 ESD Damage.
Start with a fully assembled chamber, filled with some medium, e.g. water.
- Record the Raw signal at open chamber (same conditions as at the beginning of a Sensor test. If the signal is off scale (9.99 V) try to decrease the gain (Gain = 1 for atmospheric saturation).
- Empty the chamber. Remove the POS connector plus the still attached Polarographic oxygen sensor. Leave the sensor attached to the POS connector. Set an event, describing what you did and record the signal for a short time.
- Remove the Polarographic oxygen sensor from the POS connector. Set an event, describing what you did and record the signal for a short time.
- Remove the POS connector from the Main unit. Set an event, describing what you did and record the signal for a short time.
State | Description | Expected behavior |
I | fully assembled, sensor in chamber, air saturation, Gain =2 | stable signal, 3 to 5 V |
II | POS connector + sensor removed from chamber, sensor still connected to sensor connector | initial change in voltage, new, stable non-zero level of signal reached |
III | sensor removed from POS connector | stable signal at 0 V |
IV | POS connector removed from main unit | for oxygraphs series A to C: typically signal off scale (+/- 9.99 V), for oxygraphs series D and higher: typically 0 V; may vary, a gain independent stable signal of 0.4 V is a strong indication of an electronic problem in the main unit |
State expected behavior observed? yes/no |
Interpretation / Comment | |||
---|---|---|---|---|
I | II | III | IV | |
no | yes | yes | yes | may indicate a problem in the Chamber assembly, or a defective sensor, continue testing |
no | no/yes | yes | yes | probable defect in the Sensor. This indication is not so strong as a localization obtained via switching components. The POS connector (not the Sensor) may be defect, but this may be only visible when a signal is present. |
no | no | no | yes | indicates a defect in the POS connector |
no | no | no | no | indicates a problem with Chamber assembly or a defect in the Main unit; if the problem can not be solved by reassembling the chamber, contact OROBOROS Instruments. |