Talk:O2k-Videosupport: Difference between revisions
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== Videoclip for pfi == ย | == Videoclip for pfi == ย | ||
::::# 02:00 --> When BIOPS is added to the wells (plate): add the text: all wells 2 mL BIOPS. The text will stay until: 02:10. | ::::# 02:00 --> When BIOPS is added to the wells (plate): add the text: '''all wells 2 mL BIOPS'''. The text will stay until: 02:10. | ||
::::# 02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well. | ::::# 02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well. | ||
::::# 03:00 --> Add text for the saponin concentration --> 5 mg/mL in BIOPS | ::::# 03:00 --> Add text for the saponin concentration --> '''5 mg/mL in BIOPS''' | ||
::::# 03:37 --> Add text with the information --> 20 ยตL saponin in 2 mL BIOPS | ::::# 03:37 --> Add text with the information --> '''20 ยตL saponin in 2 mL BIOPS''' | ||
::::# 03:42 --> Add text with the information --> BIOPS ( | ::::# 03:42 --> Add text with the information --> '''BIOPS''' (this text will be in the first line of the plate); '''BIOPS+saponin''' (in the second line); '''BIOPS''' (in the last line) ย | ||
::::# 04:41 --> Incorporate images from the binocular. Johannes | ::::# 04:41 --> Incorporate images from the binocular. Johannes: is maybe possible to do any animation (with the panel showing all the steps)? | ||
::::# 06:49 --> Add the text: Aluminum cup on the petri dish | ::::# 06:49 --> Add the text: '''Aluminum cup on the petri dish''' | ||
::::# 07:50 --> Add the text: Blotting during 40 seconds | ::::# 07:50 --> Add the text: '''Blotting during 40 seconds''' | ||
::::# 08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: check the forceps | ::::# 08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: '''check the forceps''' | ||
::::# 08:16 --> Add the text info?: take wet weight | ::::# 08:16 --> Add the text info?: '''take wet weight''' | ||
::::# ~08:39-08:40 --> When we take the forceps, we can see something. | ::::# ~08:39-08:40 --> When we take the forceps, we can see something. | ||
::::# ~08:56-08:59 --> Add the text: Add O<sub>2</sub> | ::::# ~08:56-08:59 --> Add the text: '''Add O<sub>2</sub>''' | ||
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== Videoclip for Shredder == ย | == Videoclip for Shredder == ย | ||
::::# ~00:19 --> Add text? Calculate volume needed for your thom | ::::# ~00:19 --> Add text? '''Calculate volume needed for your thom''' (here, pipette adding 5 mL, show pipette 5 mL (00:41)). | ||
::::# 00:41 --> Not 5 mL here, show pippete with 500 ยตL. | ::::# 00:41 --> Not 5 mL here, show pippete with 500 ยตL. | ||
::::# ~02:06 --> Text | ::::# 01:38 --> Could we incorporate a video taking the wet weight from the fiber videoclip? It will be added to the Shredder videoclip (01:38, before we see the eppendorf tube with the yellow label). We can take the video-section from the fibres (To include the sequences: 06:58-07:06; 07:12-07:13 maybe here you could do a zoom showing only the Falcon tube with the MiR05-Kit; 07:17-07:21; 07:31-07:37;ย 07:45-08:12; include the section checking the forceps from the fibres from 08:13; 08:19-08:20. Finally show the sequence 01:39 in the Shredder clip). | ||
::::# ~02:21 --> Text? | ::::# ~02:06 --> Text: '''Cut into smaller pieces''' | ||
::::# ~02:21 --> Text? '''Distributed on the Lysis Disk''' | |||
::::# ~04:02 --> 5 seconds instead of 10 seconds (in the position 2) | ::::# ~04:02 --> 5 seconds instead of 10 seconds (in the position 2) | ||
::::# ~07:33--> Not all the volume is take it. Does Johannes has another video? | ::::# ~07:33--> Not all the volume is take it. Does Johannes has another video? | ||
::::# Last part of the video is in the pfi video. | ::::# Last part of the video is in the pfi video. | ||
=== Clips for Shredder === ย | === Clips for Shredder === ย |
Revision as of 11:47, 13 February 2018
Last update: 2018-02-12
Videoclip for pfi
- 02:00 --> When BIOPS is added to the wells (plate): add the text: all wells 2 mL BIOPS. The text will stay until: 02:10.
- 02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well.
- 03:00 --> Add text for the saponin concentration --> 5 mg/mL in BIOPS
- 03:37 --> Add text with the information --> 20 ยตL saponin in 2 mL BIOPS
- 03:42 --> Add text with the information --> BIOPS (this text will be in the first line of the plate); BIOPS+saponin (in the second line); BIOPS (in the last line)
- 04:41 --> Incorporate images from the binocular. Johannes: is maybe possible to do any animation (with the panel showing all the steps)?
- 06:49 --> Add the text: Aluminum cup on the petri dish
- 07:50 --> Add the text: Blotting during 40 seconds
- 08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: check the forceps
- 08:16 --> Add the text info?: take wet weight
- ~08:39-08:40 --> When we take the forceps, we can see something.
- ~08:56-08:59 --> Add the text: Add O2
Clips for pfi
- 1. Materials (00:00-01:30)
- 2. BIOPS and saponin (01:30-03:55)
- 3. Muscle fibre preparation: Mechanical Preparation (03:55-04:57)
- 4. Muscle fibre preparation: Chemical Permeabilization (04:57-06:33)
- 5. Wet Weight (06:34-08:17)
- 6. To the O2k (08:18-09:15)
Videoclip for Shredder
- ~00:19 --> Add text? Calculate volume needed for your thom (here, pipette adding 5 mL, show pipette 5 mL (00:41)).
- 00:41 --> Not 5 mL here, show pippete with 500 ยตL.
- 01:38 --> Could we incorporate a video taking the wet weight from the fiber videoclip? It will be added to the Shredder videoclip (01:38, before we see the eppendorf tube with the yellow label). We can take the video-section from the fibres (To include the sequences: 06:58-07:06; 07:12-07:13 maybe here you could do a zoom showing only the Falcon tube with the MiR05-Kit; 07:17-07:21; 07:31-07:37; 07:45-08:12; include the section checking the forceps from the fibres from 08:13; 08:19-08:20. Finally show the sequence 01:39 in the Shredder clip).
- ~02:06 --> Text: Cut into smaller pieces
- ~02:21 --> Text? Distributed on the Lysis Disk
- ~04:02 --> 5 seconds instead of 10 seconds (in the position 2)
- ~07:33--> Not all the volume is take it. Does Johannes has another video?
- Last part of the video is in the pfi video.
Clips for Shredder
- 1. Materials (00:00-01:30)
- 2. Tissue preparation (01:30-03:02)
- 3. Homogenization (~03:02-04:27)
- 4. Retrieval (~04:27-to the end)
- 5. To the O2k (from 09:12 in the pfi video)