MiPNet06.05 Test Experiments on O2k-Specifications: Difference between revisions
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{{Publication | {{Publication | ||
|title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr | |title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5. | ||
|info=[http://www.oroboros.at/index.php?id=o2k-specifications MiPNet06.05] | |info=[http://www.oroboros.at/index.php?id=o2k-specifications MiPNet06.05] | ||
|authors=Gnaiger E, Β | |authors=Gnaiger E, | ||
|year=* | |year=* | ||
|journal=Mitochondr | |journal=Mitochondr Physiol Network | ||
|abstract=Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term βhigh-resolution respirometryβ: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 ΞΌM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 Β°C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system. | |abstract=Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term βhigh-resolution respirometryβ: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 ΞΌM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 Β°C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system. | ||
|mipnetlab=AT Innsbruck OROBOROS, | |mipnetlab=AT Innsbruck OROBOROS, |
Revision as of 11:36, 22 June 2012
Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5. |
Β» MiPNet06.05
Abstract: Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term βhigh-resolution respirometryβ: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 ΞΌM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 Β°C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.
β’ O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
HRR: Oxygraph-2k, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.