Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
-Week 32
+{{Template:Science for Ukraine}}
-* '''Aug-03 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-| '''What do you do if the values for the “O2 slope background” fields are not appearing correctly in the DatLab  “Mark statistics” window?'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-It is possible to retrieve the values and correct units displayed in the Mark Statistics window by updating the Windows control panel settings. <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- » [[MitoPedia:_DatLab#Problems_with_text_in_the_.E2.80.9CMark_Statistics.E2.80.9D_window|  Troubleshooting for Mark Statistics window]]«
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-|}
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
+----
+* '''May-21 Tu'''
 ----
-* '''Aug-04 Tu'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]«
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-* '''Aug-05 We'''
+* '''May-23 Th'''
 ----
-* '''Aug-06 Th'''
+* '''May-24 Fr'''
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
-| OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']]) describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br>
-- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«
-|}
 ----
-* '''Aug-07 Fr'''
-Week 31
+'''<big>Next Week</big>'''
+Week 22
-* '''Jul-27 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«
+<br>
-|}
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
 ----
-* '''Jul-28 Tu''' - World Hepatitis Day
+* '''May-28 Tu'''
-:{|
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
-|'''Insights into Hepatitis C virus-linked mitochondrial dysfunction''' <br>
-: from the O2k-Network »[[IT Foggia Capitanio N]]« <br>
-*           Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60.  [[Ripoli 2009 J Virol|»Bioblast link«]]
-|}
 ----
-* '''Jul-29 We'''
+* '''May-29 We'''
 ----
-* '''Jul-30 Th'''
+* '''May-30 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-- »'''[[Chlororespiration]]'''«
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Jul-31 Fr'''
+* '''May-31 Fr'''
+----