MitoKit-CII/Succinate-nv: Difference between revisions
From Bioblast
m (Zhang Feiyuan moved page NV118/MitoKit-CII to MitoKit-CII/Succinate-nv) |
No edit summary |
||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=Snv | |abbr=Snv | ||
|description=''' | |description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinae; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[succinate]] in the intracellular space. NeuroVive #: NV118 | ||
|info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | |info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | ||
}} | }} | ||
Line 9: | Line 9: | ||
== Application in HRR == | == Application in HRR == | ||
:::: ''' | :::: '''Succinate-nv:''' 25 mg, MW=262.2 g.mol<sup>-1</sup> | ||
:::: '''Preparation of 1 M stock solution:''' | :::: '''Preparation of 1 M stock solution:''' | ||
::::# Dissolve 25 mg | ::::# Dissolve 25 mg Snv in 95.3 ยตL DMSO. | ||
:::: '''Preparation of 100 mM stock solution (from the | ย | ||
:::: '''Preparation of 100 mM stock solution of Snv (from the 1 M stock):''' ย | |||
::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ||
::::# Divide into 50 ยตL portions.ย ย | ::::# Divide into 50 ยตL portions.ย ย | ||
Line 18: | Line 19: | ||
:::ยป '''O2k manual titrations''' ย | :::ยป '''O2k manual titrations''' ย | ||
::::* Event name: Snv | |||
::::* Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber). | ::::* Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 50 ยตM steps. | ::::* Final concentration: 50 ยตM steps. | ||
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. | ::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. |
Revision as of 17:10, 3 June 2019
Description
MitoKit-CII/Succinate-nv (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinae; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space. NeuroVive #: NV118
Abbreviation: Snv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Succinate-nv: 25 mg, MW=262.2 g.mol-1
- Preparation of 1 M stock solution:
- Dissolve 25 mg Snv in 95.3 ยตL DMSO.
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO.
- Divide into 50 ยตL portions.
- Store at -20 ยฐC.
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- ยป O2k manual titrations
- Event name: Snv
- Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 50 ยตM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- ยป O2k manual titrations