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== Calcium Green in high-resolution respirometry ([[HRR]]) == ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc

:::: In this configuration the O2k can be used for high-resolution respirometry and fluorometry. It is not necessary to dismount the Fluorescence-Control U ...k-Guide: Setup - next step - [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''

Application of [[permeabilized muscle fibers]] and [[high-resolution respirometry]] offer a sensitive diagnostic test of mitochondrial dysfunction in small [ ...rcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers

...can you reduce the amount of biological sample needed for high-resolution respirometry? '''<br> | '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>

»{{MitoPedia O2k and high-resolution respirometry |mitopedia O2k and high-resolution respirometry=O2k hardware

::::#Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopi ! High-resolution respirometry:

...uction using the Amplex UltraRed assay in combination with high-resolution respirometry.'''<br> | '''Do you know how to select the correct fluorescence sensors and filter set?''' <br>

...umental background oxygen flux is an essential standard in high-resolution respirometry. At the same time, an instrumental background experiment is an ultimate tes ...O₂ background test, taking care of the position of the [[TIP2k Filter Papers]]. Additionally, it is better to use the layout ''02-Calibration Bac

{{MitoPedia O2k and high-resolution respirometry}} ...Fluo-Sensor: [[Fluorescence-Sensor Green]] equipped with [[Filter_Set_AmR|Filter Set AmR]].

== Magnesium Green in high-resolution respirometry ([[HRR]]) == ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc

...on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control f ...nal (O₂ slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O₂ background test?'''<br>

::: [[Respirometry]] has been accessed more than == MiPNet discussion forum: lymphocyte isolation for respirometry (2013-03-31) ==

|''' Human [[PBMC]] and [[Platelet|Platelets]] - samples for high-resolution respirometry obtained by minimally invasive procedures.'''<br> ...can you reduce the amount of biological sample needed for high-resolution respirometry? '''<br>

:::: A Savitzky-Golay [[smoothing]] filter is used in DatLab as a basis for calculating the time derivative (Flux / Sl |mitopedia method=Respirometry, Fluorometry

...for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods En :::# Fluo-Sensor: [[Smart Fluo-Sensor Blue]] equipped with [[Filter Set Saf]]

===1. Background: High-Resolution Respirometry=== ...l of bioenergetic protocols for biomedical applications of high-resolution respirometry. The scientific publications and numerous invited lectures at international

=== Background: High-Resolution Respirometry === ...l of bioenergetic protocols for biomedical applications of high-resolution respirometry. The scientific publications and numerous invited lectures at international

...]''' - ''Unlocking cell metabolism: Exploring the power of high-resolution respirometry with the NextGen-O2k'' ...Consensus Meeting 2024 Rome IT]]''' - ''Quality-controlled high-resolution respirometry: bacterial and clinical applications''

::::» [[MitoPedia: Media for respirometry]] ...rcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers

:::: In high-resolution respirometry [[HRR]] the Amplex® UltraRed method (Mohanty et al 1997) is used with the ::::» [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging filter set of the Smart Fluo-Sensors]