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...the model analysis the metabolic fluxes are balanced. Glycolysis, pentose phosphate pathway, TCA cycle, anaplerotic reactions and transport reactions between c ...to isocitrate, as indicated by the negative value. Flux across the citrate transporter on the y-axis is out of the mitochondria, as indicated by the negative valu

::::* '''B1''', KCl-based: 130 mM potassium chloride, 5 mM dibasic potassium phosphate, 1 mM magnesium chloride, 20 mM MOPS, 1 mM EGTA, 0.1% (w/v) BSA at pH 7.1 a ...se, 60 mM potassium lactobionate, 20 mM taurine, 10 mM monobasic potassium phosphate, 3 mM magnesium chloride, 20 mM HEPES, 1 mM EGTA, and 0.1% (w/v) BSA at pH

...ex IV; Cytochrome c Oxidase •• Complex V; ATP Synthase •• Inner mtMembrane Transporter •• Marker Enzyme •• Supercomplex •• TCA Cycle and Matrix Dehydr ...e transport •• mt-Membrane potential •• Oxygen kinetics •• PCr;Cr •• pH •• Phosphate •• Redox state •• Substrate •• Temperature •• Threshold;exc

...ation system (ATP synthase, adenylate translocator and inorganic phosphate transporter). Electron flow from pyruvate&malate (PM) or glutamate&malate (GM) converge

...extra-mitochondrial concentrations of fuel substrates, ADP, ATP, inorganic phosphate, and cations including H+ can be controlled to determine mitochondrial func ...inetically saturating concentrations of fuel substrates, ADP and inorganic phosphate, and O₂, or at optimal uncoupler concentrations, respectively, i

...gating the physiological substrate and role of the circadian mitochondrial transporter Slc25a34 in brown adipose tissue. ...iffusion limitations imposed on mitochondria for oxygen and ADP, inorganic phosphate and energy substrates. To reduce these limitations, we evaluated a method f