MiPNet18.05 Amplex-Mouse-heart
O2k-Fluorometry: HRFR and H2O2 production in mouse cardiac tissue homogenate. |
ยป ยปVersions
Oroboros (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRFR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining High-Resolution FluoRespirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplexยฎ Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
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โข O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder