Palmeira 2012 Methods Mol Biol
Palmeira CM, Rolo AP (2012) Mitochondrial membrane potential (ΞΞ¨) fluctuations associated with the metabolic states of mitochondria. Methods Mol Biol 810:89-101. |
Palmeira CM, Rolo AP (2012) Methods Mol Biol
Abstract: The study of the mitochondrial membrane potential (ΞΞ¨) is essential for an integrated appraisal of mitochondrial function, since it reflects differences in electrical potential and represents the main component of the proton electrochemical gradient accounting for more than 90% of the total available respiratory energy. Numerous methods have been used to estimate mitochondrial membrane potential (ΞΞ¨), including fluorescent methods and electrochemical probes. In this chapter, we describe several practical approaches that allow mitochondrial membrane potential (ΞΞ¨) evaluation, by using a tetraphenylphosphonium (TPP(+))-selective electrode. The main focus is given to the evaluation of ΞΞ¨ in isolated mitochondria. β’ Keywords: TPP+-selective electrode, membrane potential, mitochondria, permeabilized cells, metabolic states, permeability transition β’ Bioblast editor: Sumbalova Z β’ O2k-Network Lab: PT Coimbra Laranjinha J
Labels: MiParea: Instruments;methods
Stress:Permeability transition, Oxidative stress;RONS Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Regulation: ATP production, Calcium, mt-Membrane potential, Substrate, Uncoupler, Q-junction effect
HRR: TPP, Theory