From Bioblast
Description
In respiratory assays for cytochrome c oxidase (COX; complex CIV) activity, ascorbate is added as regenerating system for reduced TMPD.
Abbreviation: As
Reference: MiPNet06.06
MitoPedia topics:
Substrate and metabolite
Application in HRR
As: Ascorbate sodium salt (Succinate disodium salt, hexahydrate, C6H706Na); Sigma A 4034, 100 g, store at R.T.; FW = 198.1
Caution: Light sensitive (store solution in a dark glass vial).
Preparation of 800 mM stock solution (dissolved in H2O):
- 1) weigh 1.584 g of Ascorbate sodium salt and dissolve in 10 ml H2O
- 2) To prevent autooxidation, adjust pH to ca 6 with ascorbic acid (a 137.6 mg ml-1 solution of pH ca 2).
- 3) Divide into 0.2 ml portions.
- 4) Store frozen at -20 Β°C.
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 Β΅l using a 25 Β΅l syringe
- Final conc. in 2 ml O2k-chamber: 2 mM