Adams 2010 Biochim Biophys Acta: Difference between revisions
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{{Publication | {{Publication | ||
|title=Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim Biophys Acta 1797: 807- | |title=Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim Biophys Acta 1797:807-16. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20417612 PMID:20417612] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20417612 PMID: 20417612 Open Access] | ||
|authors=Adams AE, Kelly OM, Porter RK | |authors=Adams AE, Kelly OM, Porter RK | ||
|year=2010 | |year=2010 | ||
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with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced โผ3-fold in UCP 1 knock-out mice compared to wild-type mice. We record a halving of CD8 single positive cell numbers in thymus with a significant incremental increase in CD4/CD8 double positives cell numbers in the thymus of UCP 1 knock-out mice compared to wild-type mice. These data are mirrored by an approximate halving of CD8 single positive cell numbers and a doubling of CD4/CD8 double positive cell numbers in the spleen of UCP 1 knock-out mice compared to wild-type mice. These differences are most probably explained by our observations of decreased apoptotic potential and higher ATP levels in thymocytes of UCP 1 knock-out mice | with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced โผ3-fold in UCP 1 knock-out mice compared to wild-type mice. We record a halving of CD8 single positive cell numbers in thymus with a significant incremental increase in CD4/CD8 double positives cell numbers in the thymus of UCP 1 knock-out mice compared to wild-type mice. These data are mirrored by an approximate halving of CD8 single positive cell numbers and a doubling of CD4/CD8 double positive cell numbers in the spleen of UCP 1 knock-out mice compared to wild-type mice. These differences are most probably explained by our observations of decreased apoptotic potential and higher ATP levels in thymocytes of UCP 1 knock-out mice | ||
when compared to wild-type controls. We conclude that constitutively expressed UCP 1 is a factor in determining T-cell population selection in mice. | when compared to wild-type controls. We conclude that constitutively expressed UCP 1 is a factor in determining T-cell population selection in mice. | ||
|keywords= | |keywords=Mitochondria, Uncoupling protein, Thymus, Spleen | ||
|mipnetlab= | |mipnetlab=IE Dublin Porter RK | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Mouse | |organism=Mouse | ||
|tissues=Islet | |tissues=Islet cell;pancreas;thymus | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 11:22, 26 May 2015
Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim Biophys Acta 1797:807-16. |
Adams AE, Kelly OM, Porter RK (2010) Biochim Biophys Acta
Abstract: Our laboratory has previously demonstrated the presence of constitutively expressed mitochondrial uncoupling protein 1 in mouse thymocytes. In our endeavours to understand the role of mitochondrial uncoupling protein 1 in thymocyte function, we compared cell profiles in thymus and spleen of wild-type with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced โผ3-fold in UCP 1 knock-out mice compared to wild-type mice. We record a halving of CD8 single positive cell numbers in thymus with a significant incremental increase in CD4/CD8 double positives cell numbers in the thymus of UCP 1 knock-out mice compared to wild-type mice. These data are mirrored by an approximate halving of CD8 single positive cell numbers and a doubling of CD4/CD8 double positive cell numbers in the spleen of UCP 1 knock-out mice compared to wild-type mice. These differences are most probably explained by our observations of decreased apoptotic potential and higher ATP levels in thymocytes of UCP 1 knock-out mice when compared to wild-type controls. We conclude that constitutively expressed UCP 1 is a factor in determining T-cell population selection in mice. โข Keywords: Mitochondria, Uncoupling protein, Thymus, Spleen
โข O2k-Network Lab: IE Dublin Porter RK
Labels:
Organism: Mouse
Tissue;cell: Islet cell;pancreas;thymus
Preparation: Intact cells
HRR: Oxygraph-2k