Ap5A: Difference between revisions

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{{Chemical_description
{{Chemical_description
|abbr=Ap5A
|abbr=Ap5A
|trivial name=Ap5a
|trivial name=Ap5A
|complete name=P1,P5-Di(adenosine-5โ€ฒ) pentaphosphate pentasodium salt
|complete name=P1,P5-Di(adenosine-5โ€ฒ) pentaphosphate pentasodium salt
|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> ยท 5 Na
|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> ยท 5 Na

Latest revision as of 10:25, 21 June 2021


high-resolution terminology - matching measurements at high-resolution


Ap5A

Description

P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ†” 2 ADP).

Abbreviation: Ap5A


Application in HRR

Ap5A: Ap5A (P1,P5-Di(adenosine-5โ€ฒ) pentaphosphate pentasodium salt; C20H29N10O22P5 ยท 5 Na), Sigma-Aldrich: D4022, store at -20 ยฐC, CAS: 4097-04-5, M = 1026.28 gยทmol-1


Preparation of 100 mM stock solution (dissolved in H2O)
  1. Dissolve content of Sigma 2 mg glass vial in 19.49 ยตL H2O
  2. Store at -20 ยฐC.
ยป O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
  • Final concentration: 50 ยตM.


References






MitoPedia topics: Inhibitor 

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