Attached cells
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The OROBOROS-O2k is principally designed to measure respiration in non-adherent, suspended samples, i.e. suspensions of cells or mitochondria, tissue homogenates or permeabilized fibers. However, successful efforts have been made to apply the OROBOROS-O2k for measurements on adherent cells. Specifically, the lab of Gregory Brewer developed techniques for high-resolution respirometry with the OROBOROS-O2k of neuronal cells attached to a substrate:
- Jones TT, Brewer GJ (2009) Critical age-related loss of cofactors of neuron cytochrome c oxidase reversed by estrogen. Exp Neurology 215: 212-219
- Jones TT, Brewer GJ (2010) Age-related deficiencies in Complex I endogenous substrate availability and reserve capacity of Complex IV in cortical neuron electron transport. Biochim Biophys Acta Bioenergetics 1797: 167-176.
