Description
Blebbistatin is a is widely used muscle and non-muscle myosin II-specific inhibitor that block contractile activity. Blebbistatin shows selectivity and high affinity for multiple class II myosins. Permeabilized muscle fibers (pfi) are sensitive to low oxygen supply due to diffusion restrictions that limit mitochondrial respiration at the core of the fiber bundle. Therefore, hyperoxic conditions are required to counteract this limitation. Further studies have shown that the addition of blebbistatin in the respiration medium prevents fiber contraction, reduces the oxygen sensitivity and allows the study of ADP kinetics in pfi at normoxic oxygen levels. Other studies described that the presence of blebbistatin does not prevent the oxygen dependence in pfi. It has to be taken into account other limitations of blebbistatin which have been described i.e. low solubility in water, cytotoxicity and phototoxicity.
Reference: Perry 2011 Biochem J
MitoPedia concepts:
MiP concept
MitoPedia topics:
Inhibitor
References
Year | Reference | Organism | Tissue;cell | |
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Doerrier 2019 MitoFit Preprint Arch EA | 2019 | Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0009 | Human | Skeletal muscle |
Perry 2011 Biochem J | 2011 | Perry CG, Kane DA, Lin CT, Kozy R, Cathey BL, Lark DS, Kane CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle. Biochem J 437:215-22. | Human Rat | Skeletal muscle |
Year | Reference | Organism | Tissue;cell | |
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Doerrier 2019 MiP2019 | 2019 | Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. | Human | Skeletal muscle |
Doerrier 2018 MiP2018a | 2018 | Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers. | Mouse | Skeletal muscle |
Bezuidenhout 2016a Abstract MitoFit Science Camp 2016 | 2016 | Comparison of oxygen dependence of respiration in permeabilized mouse skeletal muscle fibers in two respiration media, MiR06Cr and Buffer Z containing Ctl, Cr and Blebbistatin. | Mouse | Skeletal muscle |
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