D-number: Difference between revisions
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|abbr=D### | |abbr=D### | ||
|description='''D number''' is the unique code given for each [[SUIT]] protocol. In the same [[MitoPedia: SUIT |SUIT protocol]] family (SUIT-###), there might be different protocols, specifically designed for different [[sample]] type (''e.g.'', different [[mitochondrial preparations]]) or for different applications (''e.g.'', O2, [[AmR]], [[Mitochondrial membrane potential|Fluo]], [[MgG]]). Since the use of different kinds of sample or application may result in slightly different steps, each protocol receives a different D-number. | |description='''D number''' is the unique code given for each [[SUIT]] protocol. In the same [[MitoPedia: SUIT |SUIT protocol]] family (SUIT-###), there might be different protocols, specifically designed for different [[sample]] type (''e.g.'', different [[mitochondrial preparations]]) or for different applications (''e.g.'', O2, [[AmR]], [[Mitochondrial membrane potential|Fluo]], [[MgG]]). Since the use of different kinds of sample or application may result in slightly different steps, each protocol receives a different D-number. | ||
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== D-number in the SUIT | == D-number in the SUIT code == | ||
The D-number is shown at the end of the SUIT-code, ''e.g.'', SUIT-001_O2_mt_D001. | The D-number is shown at the end of the SUIT-code, ''e.g.'', SUIT-001_O2_mt_D001. | ||
For a list of SUIT protocols sorted by D-number, see the [[SUIT_protocol_library#List_of_SUIT_protocols_with_D-numbers|list of SUIT protocols with D-numbers]] | |||
For a list of SUIT protocols sorted by D-number, see the '''[[SUIT_protocol_library#List_of_SUIT_protocols_with_D-numbers|list of SUIT protocols with D-numbers]]''' | |||
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::::* [[SUIT-001 O2 ce-pce D003]] for [[living cells]] (ce) that will be further permeabilized inside the chamber (pce), with addition of [[digitonin]]; | ::::* [[SUIT-001 O2 ce-pce D003]] for [[living cells]] (ce) that will be further permeabilized inside the chamber (pce), with addition of [[digitonin]]; | ||
::::* [[SUIT-001 O2 ce-pce D004]] for [[PBMC]]s and [[platelet]]s, added in the chamber as [[living cells]] and further permeabilized inside the chamber. | ::::* [[SUIT-001 O2 ce-pce D004]] for [[PBMC]]s and [[platelet]]s, added in the chamber as [[living cells]] and further permeabilized inside the chamber. | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT concept | |mitopedia concept=SUIT concept |
Latest revision as of 14:32, 5 August 2019
Description
D number is the unique code given for each SUIT protocol. In the same SUIT protocol family (SUIT-###), there might be different protocols, specifically designed for different sample type (e.g., different mitochondrial preparations) or for different applications (e.g., O2, AmR, Fluo, MgG). Since the use of different kinds of sample or application may result in slightly different steps, each protocol receives a different D-number.
Abbreviation: D###
D-number in the SUIT code
The D-number is shown at the end of the SUIT-code, e.g., SUIT-001_O2_mt_D001.
For a list of SUIT protocols sorted by D-number, see the list of SUIT protocols with D-numbers
Example of different D-numbers with the same SUIT-number
- The SUIT-001 family comprises different related protocols, each specifically designed for different sample preparations:
- SUIT-001 O2 mt D001 for the following mitochondrial preparations: isolated mitochondria, tissue homogenates and cells that were permeabilized previously to the addition to the chamber;
- SUIT-001 O2 pfi D002 for permeabilized fibers;
- SUIT-001 O2 ce-pce D003 for living cells (ce) that will be further permeabilized inside the chamber (pce), with addition of digitonin;
- SUIT-001 O2 ce-pce D004 for PBMCs and platelets, added in the chamber as living cells and further permeabilized inside the chamber.
MitoPedia concepts: SUIT concept