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| |description='''DatLab''' is the O2k-Software for Data Acquisition & Analysis, specifically developed for [[high-resolution respirometry]] with the [[Oxygraph-2k]]. | | __TOC__ |
| The [http://www.oroboros.at/?DatLab newest DatLab version] is included in the [[O2k-Core]], and updates of DatLab are free of charge during the warranty period of the O2k. | | {{MitoPedia without banner |
| |product id=20700
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| |product type=O2k, O2k-Core
| | |description=[[Image:Logo OROBOROS-DatLab.jpg|200px|right|DatLab]]'''DatLab''' is the O2k-Software for Data Acquisition & Analysis, specifically developed for [[high-resolution respirometry]] with the O2k. |
| |info=Previous Product ID 28001-44, [http://www.oroboros.at/?DatLab DatLab @OROBOROS]
| | The newest DatLab version is '''DatLab 8''', included in the O2k-Packages. NextGen-O2k and O2k-Series J* and higher come with DatLab 8 installed on the integrated PC (Linux). DatLab 8 is required for the NextGen-O2k. DatLab 8.1 is compatible with O2k-Series (E and higher). |
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| After many years of gradual change of DatLab 4 the multiple challenges from the increased use of MultiSensor applications prompted us to develop a new generation of software for the O2k: '''DatLab 5'''
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| Because the internal structure of DatLab 4 was becoming to inflexible for new features, DatLab 5 was completely rewritten. This required also a change in the file format for DatLab files, see "Compatibility" below. By now DatLab 5.2 offers many new features like renaming plots, changingĀ units and linear regression for the calibration of the Amp channel andĀ for calculating theĀ instrumental oxygen backgroundĀ parameters. Please consult the [http://www.oroboros.at/index.php?o2k-datlab-guide DatLab Guide] for a comprehensive description for versions up till DatLab 5.1. New featuresĀ not yet included in the manuals are described below.
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| == Installing DatLab 5 ==
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| # Get Datlab5:
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| ## from www.oroboros.at: When you are eligible to a free upgrade to Datlab5 our administration ([email protected]) will supply you with a login code. Use this code to log in at [http://www.oroboros.at/index.php?id=login] and download "'''DL5Inst.exe'''" to your computer. | |
| ## from the "OROBOROS O2k-Course on HRR" (on a USB flash drive): using the file finder, click "Manual" and then use the link labeled "DL5-Install.exe" to start the installation process. (Alternatively, you can use the link in the column at the far right to navigate to the folder containing DL5Inst.exe. The full path name is "OROBOROS O2k-Course on HRR\O2k-Building Blocks\3.O2k-Manual\O2k-DatLab-Install".
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| # Start (double click) '''DL5Inst.exe'''.
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| # You may be asked to permit the installation by the Windows operating system.
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| # C:\DatLab will be suggested as installation directory. Usually this will be also be the directory used by DatLab4 and you can accept the suggestion.
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| # Select "type of installation" = "typical" this will keep your instrument specific setting (chamber labels, calibration parameters) but may overwrite some layouts.
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| # During the first start of DatLab 5 the information in any Datlab4.ini file in the same directory will be copied to Datlab5.ini, then Datlab4.ini will be deleted together with DatLab4.exe. The new executable file will be Datlab5.exe. A new desktop shortcut will be created, named "Oroboros DatLab". It may be necessary to manually delete any old shortcuts (icons) to Datlab4, if present.
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| If for some reason you want to use DatLab4 and DatLab5 on the same computer they have to be installed in two different directories.
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| Known Issues: Windows 7: When selecting an alternative directory for installing DatLab (or creating a new directory by selecting a non existing directory) Windows 7 may respond with an error message. Ignore the error message and proceed.
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| === Upgrading from DatLab 4 to DatLab 5 ===
| | The DatLab software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices. |
| DatLab 5 can beĀ installed over DatLab 4 (recommended option). In this case all DatLabĀ files will be kept, but some layouts may be overwritten.
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| | The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP (64-bit version). However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 (64-bit version) and SSD. For the proper display of DatLab on your computer, please make sure the āLanguage settingsā are set to English. |
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| == New Features in DatLab 5.2 not yet included in the Manuals ==
| | *Optionally available without integrated PC. |
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| == Compatibility with DatLab 4 == | | |info=[[Image:MitoPedia.jpg|left|60px|link=http://www.bioblast.at/index.php/MitoPedia:_DatLab |MitoPedia: DatLab]] [[MitoPedia: DatLab]] |
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| DatLabĀ 5 is downwards compatible with DatLab 4: DatLab 5 can read all filesĀ generated by DatLab 4. However, DatLab 5 will save these files always inĀ the DatLab 5 format. Afterwards, these files will no longer be readableĀ by DatLab 4. Therefore, we recommend doing a complete transition fromĀ DatLab 4 to DatLab 5, upgrading all computers. However, there is no needĀ to mass convert the old DatLab 4 files. They can stay in the DatLab 4Ā format and will be converted by DatLab 5 when necessary. If you have toĀ import DatLab 4.1 files into DatLab 5, please upgrade to DatLab 5.1.0.130 or higher.
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| | [[File:Expand.png|right|45px |Click to expand or collaps]] |
| | <div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF"> |
| | <span style="font-size:105%; color:#424242">[[File:Questions.jpg|left|18px |Asking]]'''Does [[DatLab]] software run on MAC devices?'''</span> |
| | <div class="mw-collapsible-content"> No. The [[DatLab]] software is designed for Windows operating systems. |
| | </div> |
| | </div> |
| | <br> |
| | {{Labeling |
| | |area=Instruments;methods |
| | |instruments=Oxygraph-2k, O2k-Fluorometer |
| | |additional=DatLab |
| | }} |
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| == DatLab Technical Service Pages ==
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| {{#ask: mainlabel=Title|[[Category:All]] | [[Technical service::DatLab]]| ?Technical service}} | | {{MitoPedia O2k and high-resolution respirometry |
| Ā | | |mitopedia O2k and high-resolution respirometry=O2k hardware, DatLab, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry |
| {{#set:Technical service=DatLab}}
| | }} |
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| == Version History ==
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| === DatLab 5.2 ===
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| see above
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| === DatLab 5.1.1.9 ===
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| * Main menu:Ā [Oxygraph] and [MultiSensor] were integrated into [O2k-MultiSensor].Ā [O2k-MultiSensor]/ [Calibration] (F5) will always open the CalibrationĀ Window for the active plot (O2, pX or Amp).
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| * pX:Ā [O2k-MultiSensor]/ [Calibration] : Renaming the Amp channel, selectingĀ units for the calibrated signal, setting a factor for the slopeĀ calculation, and calculating the slope from raw signal works now for theĀ pX channel analogous to the Amp channel (see above).
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| *Ā [O2k-MultiSensor]/ [Calibration]: When changing the selected unit forĀ the calibrated signal, DatLab asks whether the calibration should beĀ adjusted accordingly.
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| * Double clicking "Light on / Light off" in the O2k-Status line will switch on / off the chamber light.
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| === DatLab 5.1.0.130 ===
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| * Menu [MultiSensor]/ [MS Calibration] (Ctrl +F5):Ā For the Amp channel, the user can now set the name of the plot (as it appears e.g. in axis labeling), the unit displayed in the calibrated plot, the multiplication factor used in slope calculation, and whether the slope should be calculated from the calibrated signal or the raw signal. To use these new features: [[Image:DL5_1_0_130_Amp_calibration_user_settings.PNGā|400px|right]]
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| ** Type the desired plot name in the field "Name for Amp-channel". Avoid long names.
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| ** Choose the desired unit for the calibrated signal from the drop down menu beside "Unit". Default: "ĀµM". Changing the unit '''will not''' (in DatLab 5.1.0.130) change the calibrated values. Therefore, to avoid confusion, the unit should be chosen before performing a calibration or upgrade to DatLab 5.1.1.7, see below.
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| ** Choose the desired factor for slope calculation from the drop down menu beside "Slope factor". Default: 1000. The correct unit for the slope will be set by DatLab depending on the chosen unit for the calibrated signal and the factor for slope calculation. Changing the factor '''will''' recalculate the values for the slope plot correctly.
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| ** To calculate the slope based on the [[raw signal]], activate the checkbox "Calculate from raw signal". If viewing the raw (uncalibrated signal) it is recommended to use this option.
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| ** Click "Calibrate and Copy to Clipboard" to apply all changes.
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| * Menu [Oxygraph]/ [Stirrer test] (F9) opens a window to enter the desired duration of a [[Stirrer test]] (Default: 30 s). After clicking "OK", stirring will be deactivated in both chambers and reactivated after the chosen time.
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| * Indicator for light in chambers: If the chamber light (not to be confused with light sources for fluorescence) is on, this is indicated in the status line by the text "Light on", highlighted in yellow. If the light is off the text reads "Light off", with no highlighting. This should be helpful when the chamber light is not obviously visible due to [[Fluorescence-Sensor]]s inserted into the O2k front windows.
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| * Menu [Oxygraph]/[O2 Calibration] (F5): If the the values for R1 and R0 differ by less than 1 V a warning is displayed. This typically happens when either the same mark is chosen for R1 and R0 or when the two marks for R1 and R0 are set erroneously to the same "state" of the chamber, that is both regions are either for the "open chamber" or for the "closed chamber, no oxygen" state. Check the selected marks when seeing this warning. It is possible to ignore the warning and continue with "Calibrate and Copy to Clipboard".
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| * Menu [Oxygraph]/ [O2k Control] (F7) / [Configuration]:Ā A field "pX Reference Electrode" was added. A serial number or other designation can be entered.
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| * Menu [Experiment]/ [View protocol] (Ctrl+F3): The information is now more clearly structured to discern between parameters used duringĀ a calibration (may originate from another file, different condition) and the parameters used when the file was recorded. Both, calibration information and the information about actually used parameters were expanded for the pX and Amp channels.
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| === DatLab 5.1.0.20 ===
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| * [[file:USBĀ RS232.PNG|thumb| right]]DatLab 5 supports connecting the oxygraph to aĀ computer via the computers USB port and a standard USB cable (requiresĀ O2k-Core or O2k Series E and above). Additionally, DatLab 5 stillĀ supports connection to a computer via the RS232 port. Therefore, it isĀ possible and highly recommended to upgrade all oxygraphs in a lab toĀ DatLab 5. When connecting to the O2k for the first time, the type ofĀ connection (USB or RS232) is set by radio buttons. When connecting viaĀ the USB port any detected oxygraphs will be identified by their serialĀ number and can be selected in the drop down menu to the right of "USBĀ Port". When connecting via the RS232 port the proper serial portĀ "Com..." can be selected in the drop down menu to the right of "SerialĀ Port".
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| * DatLab 5 allows to open DatLab files by double clickingĀ them in the Windows file manager or similar programs. First time use:Ā Double click any DatLab file in the Windows file manager, select "openĀ this file type always with this program", select "browse", then navigateĀ to the fileĀ Datlab5.exe. Usually this file will be inĀ C:\Datlab\DatLab5.exe. Depending on your Windows settings the extensionĀ '.exe' may not be displayed: In this case you can differentiate betweenĀ DatLab5.exe and DatLab5.ini by right clicking and selectingĀ "properties". The exact procedure will depend on the version andĀ language of your Windows operating system. After doing this once,Ā Windows will always use DatLab 5 to open DatLab files.
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| * MenuĀ [Graph]/[Scaling] (F6): It is possible to set the maximum value for the YĀ axis of a plot. When changing one or two parameters (minimum, maximum,Ā or range) the remaining parameters will be adjusted automatically afterĀ pressing "OK" or clicking in a different field.Ā Fill out only theĀ fields Minimum and Range to simulate the behavior of DatLab 4.
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| *Ā Menu [Graph]/[Scaling] (F6):It is possible to selectively copy scalingĀ settings to other windows by using the "Apply to Graph 1", Apply toĀ Graph 2", etc buttons. These buttons replace the "Apply to all" button.Ā This will be helpful in MultiSensor applications when e.g. two windowsĀ should show oxygen plots (with identical scaling) and two windows shouldĀ show fluorescence plots (with identical scaling).
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| * The numberĀ of points used for slope calculations can now be changed for the pX andĀ the Amp channels. Select [MultiSensor]/[MS Calibration]: Slope N: SelectĀ the number of data points (N = 40 to NĀ = 5 in intervals of 5) used toĀ calculate the slope. A high value of N yields a highly smoothed curve,Ā whereas a low value of N improves time resolution.
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| * The slopesĀ for the pX and Amp channel are now calculated with the correct sign forĀ the first order time derivative. That is, if the calibrated signalĀ decreases the slope will have a negative sign, if it increases the signĀ of the corresponding slope will be positive. The slope for the O2Ā channels is still calculated to give a positive sign for a decreasingĀ signal, signifying a "positive " respiration.
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| * Proton production rates can be calculated on line. In the menu select [Plots]/[Proton Flux], see [[O2k-pH ISE-Module]].
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| *The amperometric channel that, due to its main use for "NO electrodes", was labeled "NO" in DatLab 4 was renamed to "Amp" to reflect its versatile nature. It is used e.g. to record the data from the [[O2k-Fluorescence LED2-Module]].
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| * Faster processing of DatLab Data (this required a larger minimum file size).
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| __SHOWFACTBOX__
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| {{Technical service}}
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MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k.
The newest DatLab version is DatLab 8, included in the O2k-Packages. NextGen-O2k and O2k-Series J* and higher come with DatLab 8 installed on the integrated PC (Linux). DatLab 8 is required for the NextGen-O2k. DatLab 8.1 is compatible with O2k-Series (E and higher).
The DatLab software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP (64-bit version). However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 (64-bit version) and SSD. For the proper display of DatLab on your computer, please make sure the āLanguage settingsā are set to English.
*Optionally available without integrated PC.
Reference:
MitoPedia: DatLab
Does DatLab software run on MAC devices?
No. The
DatLab software is designed for Windows operating systems.
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Fluorometer
DatLab
MitoPedia O2k and high-resolution respirometry:
O2k hardware,
DatLab,
O2k-Open Support,
O2k-Respirometry,
O2k-FluoRespirometry
