Difference between revisions of "Du 1999 Free Radic Biol Med"
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{{Publication | {{Publication | ||
|title=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27: 596-604. | |title=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by ''in vitro'' anoxia/reoxygenation. Free Radic Biol Med 27:596-604. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/10490280 PMID: 10490280] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/10490280 PMID: 10490280] | ||
|authors=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE | |authors=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE | ||
|year=1999 | |year=1999 | ||
|journal=Free Radic Biol Med | |journal=Free Radic Biol Med | ||
|abstract=The present study investigated the protective effects of ''Ginkgo biloba'' extract (EGb 761) on rat liver mitochondrial damage induced by ''in vitro'' anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 ± 0.11) decreased after anoxia/reoxygenation (1.75 ± 0.09, p < .01), as well as state 3 and uncoupled respiration (−20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50–200 μg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 μg/ml EGb 761, they showed an increase in ADP/O (2.09 ± 0.14, p < .05) and a decrease in state 4 respiration (−22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. | |abstract=The present study investigated the protective effects of ''Ginkgo biloba'' extract (EGb 761) on rat liver mitochondrial damage induced by ''in vitro'' anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 ± 0.11) decreased after anoxia/reoxygenation (1.75 ± 0.09, ''p'' < .01), as well as state 3 and uncoupled respiration (−20%, ''p'' < .01), but state 4 respiration increased (''p'' < .01). EGb 761 (50–200 μg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 μg/ml EGb 761, they showed an increase in ADP/O (2.09 ± 0.14, ''p'' < .05) and a decrease in state 4 respiration (−22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. | ||
|keywords=''Ginkgo biloba'' extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR,Free radicals | |keywords=''Ginkgo biloba'' extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR,Free radicals | ||
|mipnetlab= | |mipnetlab=BE Liege Votion DM | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|injuries=Ischemia-reperfusion | |||
|organism=Rat | |||
|tissues=Liver | |||
|preparations=Isolated mitochondria | |||
|couplingstates=OXPHOS, ET | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 17:51, 9 November 2017
| Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27:596-604. |
Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) Free Radic Biol Med
Abstract: The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 ± 0.11) decreased after anoxia/reoxygenation (1.75 ± 0.09, p < .01), as well as state 3 and uncoupled respiration (−20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50–200 μg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 μg/ml EGb 761, they showed an increase in ADP/O (2.09 ± 0.14, p < .05) and a decrease in state 4 respiration (−22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. • Keywords: Ginkgo biloba extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR, Free radicals
• O2k-Network Lab: BE Liege Votion DM
Labels:
Stress:Ischemia-reperfusion Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Coupling state: OXPHOS, ET
HRR: Oxygraph-2k
