Difference between revisions of "Du 1999 Free Radic Biol Med"
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{{Publication | {{Publication | ||
|title=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27: 596-604. | |title=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27:596-604. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/10490280 PMID: 10490280] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/10490280 PMID: 10490280] | ||
|authors=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE | |authors=Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE | ||
| Line 12: | Line 12: | ||
|organism=Rat | |organism=Rat | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|injuries=Ischemia- | |injuries=Ischemia-reperfusion;preservation | ||
|couplingstates=OXPHOS, ETS | |couplingstates=OXPHOS, ETS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 13:08, 13 February 2015
| Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27:596-604. |
Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) Free Radic Biol Med
Abstract: The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 Β± 0.11) decreased after anoxia/reoxygenation (1.75 Β± 0.09, p < .01), as well as state 3 and uncoupled respiration (β20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50β200 ΞΌg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 ΞΌg/ml EGb 761, they showed an increase in ADP/O (2.09 Β± 0.14, p < .05) and a decrease in state 4 respiration (β22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. β’ Keywords: Ginkgo biloba extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR, Free radicals
β’ O2k-Network Lab: BE_Liege_Votion DM
Labels:
Stress:Ischemia-reperfusion;preservation"Ischemia-reperfusion;preservation" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
