Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Du 1999 Free Radic Biol Med

From Bioblast
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Publications in the MiPMap
Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27:596-604.

» PMID: 10490280

Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) Free Radic Biol Med

Abstract: The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 ± 0.11) decreased after anoxia/reoxygenation (1.75 ± 0.09, p < .01), as well as state 3 and uncoupled respiration (−20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50–200 μg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 μg/ml EGb 761, they showed an increase in ADP/O (2.09 ± 0.14, p < .05) and a decrease in state 4 respiration (−22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. Keywords: Ginkgo biloba extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR, Free radicals

O2k-Network Lab: BE Liege Votion DM


Labels:

Stress:Ischemia-reperfusion  Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria 


Coupling state: OXPHOS, ET 

HRR: Oxygraph-2k