Furlanetto 2018 Physiol Plant: Difference between revisions
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{{Publication | {{Publication | ||
|title=Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Møller IM (2018) Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells. Physiol Plant | |title=Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Møller IM (2018) Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells. Physiol Plant 166:513-24. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/29952010 PMID: 29952010] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/29952010 PMID: 29952010] | ||
|authors=Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Moeller IM | |authors=Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Moeller IM | ||
|year=2018 | |year=2018 | ||
|journal=Physiol Plant | |journal=Physiol Plant | ||
|abstract=We evaluated the effect of global warming on ''Araucaria angustifolia'' (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high-temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6h, respectively, while 2h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2h at 42°C. The ''in vitro'' activity of enzymes of the base excision repair (BER) pathway was determined. AP endonuclease, measured in extracts from cells incubated for 2h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30 min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited by 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and | |abstract=We evaluated the effect of global warming on ''Araucaria angustifolia'' (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high-temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6h, respectively, while 2h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2h at 42°C. The ''in vitro'' activity of enzymes of the base excision repair (BER) pathway was determined. AP endonuclease, measured in extracts from cells incubated for 2h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30 min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited by 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, ''A. angustifolia'' will likely be vulnerable to the short-term high-temperature events associated with global warming. | ||
<small>This article is protected by copyright. All rights reserved.</small> | <small>This article is protected by copyright. All rights reserved.</small> | ||
| Line 14: | Line 14: | ||
|injuries=Temperature | |injuries=Temperature | ||
|organism=Plants | |organism=Plants | ||
|preparations= | |preparations=Intact cells | ||
|couplingstates=ET | |couplingstates=ROUTINE, ET | ||
|pathways=N, CIV, ROX | |pathways=N, CIV, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels, 2018-08, | |additional=Labels, 2018-08, | ||
}} | }} | ||
Latest revision as of 12:10, 9 July 2019
| Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Møller IM (2018) Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells. Physiol Plant 166:513-24. |
Furlanetto ALDM, Cadena SMSC, Martinez GR, Ferrando B, Stevnsner T, Moeller IM (2018) Physiol Plant
Abstract: We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high-temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6h, respectively, while 2h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. AP endonuclease, measured in extracts from cells incubated for 2h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30 min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited by 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, A. angustifolia will likely be vulnerable to the short-term high-temperature events associated with global warming.
This article is protected by copyright. All rights reserved.
• Bioblast editor: Plangger M, Kandolf G
Labels: MiParea: Respiration
Stress:Temperature Organism: Plants
Preparation: Intact cells
Coupling state: ROUTINE, ET
Pathway: N, CIV, ROX
HRR: Oxygraph-2k
Labels, 2018-08
