Glucose: Difference between revisions
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::1) weigh 360.32 mg of Glucose ย | ::1) weigh 360.32 mg of Glucose ย | ||
::2) dissolve 1 ml H<sub>2</sub>O in 1, | ::2) dissolve 1 ml H<sub>2</sub>O in 1,5 ml Eppendorf | ||
'''Oxygraph-2k Titration:'''ย ย | '''Oxygraph-2k Titration:'''ย ย | ||
Line 24: | Line 24: | ||
::* Titration volume: 20 ยตl using a 50 ยตl syringe | ::* Titration volume: 20 ยตl using a 50 ยตl syringe | ||
::* Final conc. in 2 ml O2k-chamber: 20 mM | ::* Final conc. in 2 ml O2k-chamber: 20 mM | ||
'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::1) weigh 79.2 mg of Glucose | |||
::2) dissolve 0.2 ml H<sub>2</sub>O in 0,5 ml Eppendorf | |||
'''Oxygraph-2k Titration:'''ย | |||
::* Titration volume: 5 ยตl using a 25 ยตl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L) | |||
::* Titration volume: 10 ยตl using a 25 ยตl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L) |
Revision as of 13:47, 18 May 2017
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
MitoPedia topics: Substrate and metabolite
Application in HRR
Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at R.T.; FW = 180.16
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 360.32 mg of Glucose
- 2) dissolve 1 ml H2O in 1,5 ml Eppendorf
Oxygraph-2k Titration:
- Titration volume: 20 ยตl using a 50 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 20 mM
Preparation of 2.2 M stock solution (dissolved in H2O):
- 1) weigh 79.2 mg of Glucose
- 2) dissolve 0.2 ml H2O in 0,5 ml Eppendorf
Oxygraph-2k Titration:
- Titration volume: 5 ยตl using a 25 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L)
- Titration volume: 10 ยตl using a 25 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L)