Gnaiger 2020 BEC MitoPathways: Difference between revisions
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* '''Preface''' | * '''Preface''' | ||
:: '''References Preface''' | |||
== Chapter 1. OXPHOS analysis == | == Chapter 1. OXPHOS analysis == | ||
=== References Chapter 1 === | === References Chapter 1 === | ||
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=== References Chapter 2 === | === References Chapter 2 === | ||
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=== References Chapter 3 === | === References Chapter 3 === | ||
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== Chapter 5. Respiratory states, coupling control and coupling control ratios | Β | ||
== Chapter 5. Respiratory states, coupling control and coupling control ratios == | |||
=== References Chapter 5 === | === References Chapter 5 === | ||
== Chapter 6. Conversions of metabolic fluxes | Β | ||
== Chapter 6. Conversions of metabolic fluxes == | |||
=== References Chapter 6 === | === References Chapter 6 === |
Revision as of 09:23, 25 November 2012
Gnaiger E ed (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. Mitochondr Physiol Network 17.##: ## pp. 3rd ed. ISBN 978-3-9502399-6-6 |
Β» MiPNet2007
Gnaiger E (2012)
Abstract: β’ Keywords: ETS, Q-junction, respiratory states, flux control ratios
β’ O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS
Labels:
Organism: Human, Mouse
Tissue;cell: Skeletal muscle, Fibroblast
Preparation: Permeabilized cells, Permeabilized tissue, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Theory, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Supplementary information
- Preface
- References Preface
Chapter 1. OXPHOS analysis
References Chapter 1
Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate
Notes - Pitfalls
- Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. βOf the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..β
- Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited.
- Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of βsparking malate concentrationsβ. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 Β΅M malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees.
References Chapter 2
Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein
Notes - Pitfalls
- Ponsot et al (2005) J Cell Physiol 203: 479-486. β.. the mitochondrial form of GPDH, which produces FADH2 within the mitochondrial matrix and provides electrons to Compoex II of the phosphorylation chainβ. β The mitochondrial glycerophosphate dehydrogenase (GpDH), located on the outer side of the inner mitochondrial membrane, does not provide electrons to CII, but feeds electrons into the Q-cycle entirely independent of CII. FADH2 is not produced within the mitochondrial matrix. Electron transfer takes place from the mitochondrial inner membrane flavoprotein-linked glycerophosphate dehydrogenase to CoQ.
- In the first edition of βMitochondrial Pathways and Respiratory Controlβ (2007), the term βelectron transportβ is used synonymously for βelectron transferβ.