Difference between revisions of "Gnaiger 2022 Abstract Bioblast-PB"
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[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | [[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | ||
{{Abstract | {{Abstract | ||
|title=[[File:Erich Gnaiger.jpg|left|100px|Erich Gnaiger]] Went N, | |title=P03. [[File:Erich Gnaiger.jpg|left|100px|Erich Gnaiger]] Went N, Di Marcello M, <u>Gnaiger Erich</u> (2022) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by high-resolution PhotoRespirometry. '''Bioblast 2022: BEC Inaugural Conference.''' In: https://doi.org/10.26124/bec:2022-0001 <br>[[Went 2021 MitoFit PB|»''MitoFit Preprint''«]] | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Went Nora, | |authors=Went Nora, Marcello M, Gnaiger Erich | ||
|year=2022 | |year=2022 | ||
|event=Bioblast 2022 | |event=Bioblast 2022 | ||
|abstract=Algal biotechnology has emerged as a high-potential industry for efficient and CO<sub>2</sub>-neutral production of biomass providing biofuels, food and feed, and a variety of carbon-based chemicals and pharmaceuticals. Algal metabolism is directly involved in the regulation of growth, cell concentration, and biosynthesis of biotechnologically-relevant phytochemicals such as vitamins, antioxidants, and immune response boosters. Photoautotrophic growth rates of algae are based on light-to-chemical energy conversion and CO<sub>2</sub> fixation, and any optimization of biomass production requires maximizing energy-use efficiency of photosynthesis and respiration, both of which vary as a function of light intensity. As such, the bioenergetic crosstalk between mitochondria and chloroplasts plays a key role in maintaining metabolic integrity and controlling intermediary metabolite production. Â | |abstract=Algal biotechnology has emerged as a high-potential industry for efficient and CO<sub>2</sub>-neutral production of biomass providing biofuels, food and feed, and a variety of carbon-based chemicals and pharmaceuticals. Algal metabolism is directly involved in the regulation of growth, cell concentration, and biosynthesis of biotechnologically-relevant phytochemicals such as vitamins, antioxidants, and immune response boosters. Photoautotrophic growth rates of algae are based on light-to-chemical energy conversion and CO<sub>2</sub> fixation, and any optimization of biomass production requires maximizing energy-use efficiency of photosynthesis and respiration, both of which vary as a function of light intensity. As such, the bioenergetic crosstalk between mitochondria and chloroplasts plays a key role in maintaining metabolic integrity and controlling intermediary metabolite production. Â | ||
In the present study, we investigated how photosynthetic O<sub>2</sub> production and respiratory O<sub>2</sub> consumption was influenced as a function of light intensity, O<sub>2</sub> concentration and culture density in the unicellular model green alga ''Chlamydomonas reinhardtii''. Cultures were grown photoautotrophically in a modified Tris-Phosphate growth medium (TRIS, N- and P-nutrient replete) at 25 °C, pH 7.0, and light intensity of 100 ”mol photons·s<sup>-1</sup>·m<sup>-2</sup> (16:8 h light:dark cycle). Kinetics of light-induced O<sub>2</sub> production and dark respiration of these microalgae was measured under culture conditions and three cell concentrations, while varying O<sub>2</sub> concentrations in the Oroboros [[NextGen-O2k]] equipped with the PhotoBiology-Module [1] during stepwise increases of blue actinic light from from 10 to 350 ”molâs<sup>-1</sup>âm<sup>-2</sup>, followed by darkness, again at various controlled O<sub>2</sub> concentrations. Maximum net photosynthesis was inhibited by 40 % at hyperoxic O<sub>2</sub> concentrations of 550 to 650 ”M, when ROS production is known to be increased [2]. Transient light-enhanced dark respiration [ | In the present study, we investigated how photosynthetic O<sub>2</sub> production and respiratory O<sub>2</sub> consumption was influenced as a function of light intensity, O<sub>2</sub> concentration, and culture density in the unicellular model green alga ''Chlamydomonas reinhardtii''. Cultures were grown photoautotrophically in a modified Tris-Phosphate growth medium (TRIS, N- and P-nutrient replete) at 25 °C, pH 7.0, and light intensity of 100 ”mol photons·s<sup>-1</sup>·m<sup>-2</sup> (16:8 h light:dark cycle). Kinetics of light-induced O<sub>2</sub> production and dark respiration of these microalgae was measured under culture conditions and three cell concentrations, while varying O<sub>2</sub> concentrations in the Oroboros [[NextGen-O2k]] equipped with the PhotoBiology-Module [1] during stepwise increases of blue actinic light from from 10 to 350 ”molâs<sup>-1</sup>âm<sup>-2</sup>, followed by darkness, again at various controlled O<sub>2</sub> concentrations. Maximum net photosynthesis was inhibited by 40 % at hyperoxic O<sub>2</sub> concentrations of 550 to 650 ”M, when ROS production is known to be increased [2,3]. Transient light-enhanced dark respiration [4] peaked within 30 to 60 s after light-dark transitions and was 3.5- to 4-fold higher than steady-state dark respiration independent of O<sub>2</sub> concentration in the range of 200 to 650 ”M.  | ||
We conclude that high-resolution photorespiratory analysis provides a new method to investigate the oxygen kinetics of O<sub>2</sub> production and O<sub>2</sub> consumption that reveal interactions of chloroplasts and mitochondria under precisely regulated experimental light and oxygen regimes. | We conclude that high-resolution photorespiratory analysis provides a new method to investigate the oxygen kinetics of O<sub>2</sub> production and O<sub>2</sub> consumption that reveal interactions of chloroplasts and mitochondria under precisely regulated experimental light and oxygen regimes. | ||
<small> | <small> | ||
# Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry | # Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. https://doi.org/10.26124/mitofit:2021-0005 | ||
# KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed | # KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004 | ||
# Shimakawa G, Kohara A, Miyake C (2020) Characterization of light-enhanced respiration in cyanobacteria | # Shimakawa G, Kohara A, Miyake C (2020) Characterization of light-enhanced respiration in cyanobacteria. https://doi.org/10.3390/ijms22010342 | ||
</small> | </small> | ||
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}} | }} | ||
== Affiliations and support == | == Affiliations and support == | ||
:::: Went | :::: Went N, Di Marcello M, Gnaiger Erich | ||
:::: | :::: Oroboros Instruments GmbH, Innsbruck, Austria | ||
:::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionâs Horizon 2020 research and innovation programme under grant agreement nÂș 859770 | :::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionâs Horizon 2020 research and innovation programme under grant agreement nÂș 859770. | ||
== List of abbreviations, terms and definitions - MitoPedia == | == List of abbreviations, terms and definitions - MitoPedia == | ||
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{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Instruments;methods, Comparative MiP;environmental MiP | ||
|organism=Algae | |||
|preparations=Intact cells | |||
|topics=Oxygen kinetics | |||
|couplingstates=ROUTINE | |||
|instruments=Oxygraph-2k, NextGen-O2k | |instruments=Oxygraph-2k, NextGen-O2k | ||
|additional=Chlamydomonas, LEDR, Photosynthesis, | |||
|event=Poster | |||
|articletype=Abstract | |articletype=Abstract | ||
}} | }} |
Latest revision as of 14:13, 17 August 2023
P03. Went N, Di Marcello M, Gnaiger Erich (2022) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by high-resolution PhotoRespirometry. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 »MitoFit Preprint« |
Link: Bioblast 2022: BEC Inaugural Conference
Went Nora, Marcello M, Gnaiger Erich (2022)
Event: Bioblast 2022
Algal biotechnology has emerged as a high-potential industry for efficient and CO2-neutral production of biomass providing biofuels, food and feed, and a variety of carbon-based chemicals and pharmaceuticals. Algal metabolism is directly involved in the regulation of growth, cell concentration, and biosynthesis of biotechnologically-relevant phytochemicals such as vitamins, antioxidants, and immune response boosters. Photoautotrophic growth rates of algae are based on light-to-chemical energy conversion and CO2 fixation, and any optimization of biomass production requires maximizing energy-use efficiency of photosynthesis and respiration, both of which vary as a function of light intensity. As such, the bioenergetic crosstalk between mitochondria and chloroplasts plays a key role in maintaining metabolic integrity and controlling intermediary metabolite production.
In the present study, we investigated how photosynthetic O2 production and respiratory O2 consumption was influenced as a function of light intensity, O2 concentration, and culture density in the unicellular model green alga Chlamydomonas reinhardtii. Cultures were grown photoautotrophically in a modified Tris-Phosphate growth medium (TRIS, N- and P-nutrient replete) at 25 °C, pH 7.0, and light intensity of 100 ”mol photons·s-1·m-2 (16:8 h light:dark cycle). Kinetics of light-induced O2 production and dark respiration of these microalgae was measured under culture conditions and three cell concentrations, while varying O2 concentrations in the Oroboros NextGen-O2k equipped with the PhotoBiology-Module [1] during stepwise increases of blue actinic light from from 10 to 350 ”molâs-1âm-2, followed by darkness, again at various controlled O2 concentrations. Maximum net photosynthesis was inhibited by 40 % at hyperoxic O2 concentrations of 550 to 650 ”M, when ROS production is known to be increased [2,3]. Transient light-enhanced dark respiration [4] peaked within 30 to 60 s after light-dark transitions and was 3.5- to 4-fold higher than steady-state dark respiration independent of O2 concentration in the range of 200 to 650 ”M.
We conclude that high-resolution photorespiratory analysis provides a new method to investigate the oxygen kinetics of O2 production and O2 consumption that reveal interactions of chloroplasts and mitochondria under precisely regulated experimental light and oxygen regimes.
- Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. https://doi.org/10.26124/mitofit:2021-0005
- KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004
- Shimakawa G, Kohara A, Miyake C (2020) Characterization of light-enhanced respiration in cyanobacteria. https://doi.org/10.3390/ijms22010342
âą O2k-Network Lab: AT Innsbruck Oroboros
Affiliations and support
- Went N, Di Marcello M, Gnaiger Erich
- Oroboros Instruments GmbH, Innsbruck, Austria
- This work was part of the Oroboros NextGen-O2k project, with funding from the European Unionâs Horizon 2020 research and innovation programme under grant agreement nÂș 859770.
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP
Organism: Algae
Preparation: Intact cells
Regulation: Oxygen kinetics Coupling state: ROUTINE
HRR: Oxygraph-2k, NextGen-O2k Event: Poster Chlamydomonas, LEDR, Photosynthesis