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Huete-Ortega M 2021 MitoFit Dark respiration

From Bioblast
Publications in the MiPMap
Huete-Ortega M, Di Marcello M, Gnaiger E (2021) Substrate-uncoupler-inhibitor-titration protocols for dark respiration in Chlamydomonas reinhardtii. MitoFit Preprints 2021 (in prep).



Abstract:


Labels: MiParea: Respiration, Instruments;methods 



Preparation: Intact organism 

Regulation: Coupling efficiency;uncoupling  Coupling state: ROUTINE, ET  Pathway: CIV, ROX  HRR: Oxygraph-2k, NextGen-O2k 

Algae, Dark respiration, Alternative oxidase, SUIT-003, SUIT-003 O2 ce D039, SUIT-022 O2 ce D051, SUIT-023 O2 ce D053, MitoFit 2021 Photosynthesis 

ORCID: ORCID.png Huete-Ortega Maria, ORCID.png Di Marcello Marco, ORCID.png Gnaiger Erich

References

LinkViewReferenceYear
PMID:23354456Antal TK, Kukarskikh GP, Bulychev AA, Tyystjรคrvi E, Krendeleva T (2013) Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains. Planta 237:1241-1250.2013
PMID:9878636Duby F, Matagne R (1999) Alteration of dark respiration and reduction of phototrophic growth in a mitochondrial DNA deletion mutant of Chlamydomonas lacking cob, nd4, and the 3โ€™ end of nd5. Plant Cell 11:115-125.1999
Bioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.2008
High-resolution mitochondrial oxygen kinetics as diagnostic tool in Complex IV impairments.
PMID:29850998Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.2018
[1]Peltier G, Sarrey F (1988) The Kok effect and the light-inhibition of chlororespiration in Chlamydomonas reinhardtii. FEB 228:259-262.1988
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O2k-Manual
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
2023-02-06

Keywords

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Cited by

  • Huete-Ortega et al (2021) Substrate-uncoupler-inhibitor-titration protocols for photosynthesis in Chlamydomonas reinhardtii. MitoFit Preprints 2021 (in prep).