Difference between revisions of "Kluckova 2015 Methods Mol Biol"
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assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted | assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted | ||
to mitochondria on this process, and also how to assess this in tumor tissue. | to mitochondria on this process, and also how to assess this in tumor tissue. | ||
|keywords=Mitochondrial respiration , Cultured cells , Tumors , Mitochondria-targeted anticancer agents | |keywords=Mitochondrial respiration , Cultured cells , Tumors , Mitochondria-targeted anticancer agents, Mouse mesothelioma tumor cells AE 17 cells | ||
|mipnetlab=CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J | |mipnetlab=CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods, mt- | |area=Respiration, Instruments;methods, mt-Medicine, Pharmacology;toxicology | ||
|preparations=Intact cells, Permeabilized cells | |organism=Mouse | ||
|tissues=Endothelial;epithelial;mesothelial cell, Genital | |||
|model cell lines=Other cell lines | |||
|preparations=Intact cells, Permeabilized cells, Homogenate | |||
|diseases=Cancer | |diseases=Cancer | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII | |substratestates=CI, CII, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=PBI-Shredder, Review | ||
}} | }} |
Revision as of 11:19, 16 March 2016
Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. |
Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Methods Mol Biol
Abstract: Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue. β’ Keywords: Mitochondrial respiration, Cultured cells, Tumors, Mitochondria-targeted anticancer agents, Mouse mesothelioma tumor cells AE 17 cells
β’ O2k-Network Lab: CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Pharmacology;toxicology
Pathology: Cancer
Organism: Mouse Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital Preparation: Intact cells, Permeabilized cells, Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
PBI-Shredder, Review