Difference between revisions of "Kluckova 2015 Methods Mol Biol"
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{{Publication | {{Publication | ||
|title=Kluckova K, Dong | |title=Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25634276 PMID:25634276] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/25634276 PMID:25634276] | ||
|authors=Kluckova K, Dong | |authors=Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J | ||
|year=2015 | |year=2015 | ||
|journal=Methods Mol Biol | |journal=Methods Mol Biol | ||
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assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted | assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted | ||
to mitochondria on this process, and also how to assess this in tumor tissue. | to mitochondria on this process, and also how to assess this in tumor tissue. | ||
|keywords=Mitochondrial respiration , Cultured cells , Tumors , Mitochondria-targeted anticancer agents | |keywords=Mitochondrial respiration , Cultured cells , Tumors , Mitochondria-targeted anticancer agents, Mouse mesothelioma tumor cells AE 17 cells | ||
|mipnetlab=CZ Prague Neuzil J | |mipnetlab=CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods | |area=Respiration, Instruments;methods, mt-Medicine, Pharmacology;toxicology | ||
|diseases=Cancer | |diseases=Cancer | ||
| | |organism=Mouse | ||
|couplingstates=LEAK, OXPHOS, | |tissues=Endothelial;epithelial;mesothelial cell, Genital, Other cell lines | ||
| | |preparations=Intact cells, Permeabilized cells, Homogenate | ||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=PBI-Shredder, Review | ||
}} | }} | ||
Latest revision as of 14:56, 13 November 2017
| Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. |
Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Methods Mol Biol
Abstract: Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue. • Keywords: Mitochondrial respiration, Cultured cells, Tumors, Mitochondria-targeted anticancer agents, Mouse mesothelioma tumor cells AE 17 cells
• O2k-Network Lab: CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Pharmacology;toxicology
Pathology: Cancer
Organism: Mouse Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital, Other cell lines Preparation: Intact cells, Permeabilized cells, Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
PBI-Shredder, Review
