Kluckova 2015 Methods Mol Biol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Kluckova K, Dong | |title=Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25634276 PMID:25634276] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/25634276 PMID:25634276] | ||
|authors=Kluckova K, Dong | |authors=Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J | ||
|year=2015 | |year=2015 | ||
|journal=Methods Mol Biol | |journal=Methods Mol Biol |
Revision as of 14:50, 8 June 2015
Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. |
Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Methods Mol Biol
Abstract: Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue. โข Keywords: Mitochondrial respiration, Cultured cells, Tumors, Mitochondria-targeted anticancer agents
โข O2k-Network Lab: CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J
Labels: MiParea: Respiration, Instruments;methods, mt-Membrane, mt-Medicine, Patients
Pathology: Cancer
Preparation: Intact cells, Permeabilized cells
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
Labels