Difference between revisions of "Liver mitochondria purification"
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|description=[[Armstrong 2010 JCP]]: This paper describes a method for purification of rodent liver mitochdondria using relatively low-speed centrifugation through discontinuous Percoll gradients. | |description=[[Armstrong 2010 JCP]]: This paper describes a method for purification of rodent liver mitochdondria using relatively low-speed centrifugation through discontinuous Percoll gradients. | ||
|info=Armstrong, | |info=Armstrong C, Staples JF (2010) The role of succinate dehydrogenase and oxaloacetate in mitochondrial metabolism during mammalian hibernation and arousal. J. Comp. Physiol. B. 180: 775-783. | ||
}} | }} | ||
{{MitoPedia topics}} | |||
{{Labeling | {{Labeling | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
Revision as of 09:25, 15 August 2011
- high-resolution terminology - matching measurements at high-resolution
Liver mitochondria purification
Description
Armstrong 2010 JCP: This paper describes a method for purification of rodent liver mitochdondria using relatively low-speed centrifugation through discontinuous Percoll gradients.
Reference: Armstrong C, Staples JF (2010) The role of succinate dehydrogenase and oxaloacetate in mitochondrial metabolism during mammalian hibernation and arousal. J. Comp. Physiol. B. 180: 775-783.
Labels:
Organism: Rat, Other Mammal"Other Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
