Difference between revisions of "MiPNet03.02 Chemicals-Media"
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== Supplementary information == | == Supplementary information == | ||
::::» [[MiPNet09.12 O2k-Titrations]]; [[MitoPedia]] | ::::» [[MiPNet09.12 O2k-Titrations]]; [[MitoPedia]] | ||
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{{Template:Cited by Cardoso 2021 MitoFit MgG}} | {{Template:Cited by Cardoso 2021 MitoFit MgG}} | ||
{{Template:Cited by Komlodi 2021 MitoFit CoQ}} | {{Template:Cited by Komlodi 2021 MitoFit CoQ}} | ||
{{Labeling | |||
|area=Instruments;methods | |||
|instruments=O2k-Protocol | |||
|additional=O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG, MitoFit 2021 CoQ | |||
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Revision as of 22:43, 16 February 2021
MiPNet03.02 Chemicals-Media
| Selected media and chemicals for respirometry with mitochondrial preparations. |
» 
»Versions
Oroboros (2016-08-30) Mitochondr Physiol Network
Abstract: Fontana-Ayoub M, Fasching M, Gnaiger E (2016) Selected media and chemicals for respirometry with mitochondrial preparations. Mitochondr Physiol Network 03.02(18):1-10. Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of results obtained in different laboratories. Efforts towards standardization are important for the advancement of mitochondrial physiology.
- » Product: Oroboros O2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck Oroboros
Supplementary information
- » MiPNet09.12 O2k-Titrations; MitoPedia
- » Cytochrome c control factor
- » For calculations, see Excel file: O2k-Titrations.xls
- » For pH adjustment of BIOPS solution, note the temperature dependence:
- at 0 °C pH = 7.1
- at 23 °C pH = 6.75
Cited by
- Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
- Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
Labels: MiParea: Instruments;methods
HRR: O2k-Protocol
O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG, MitoFit 2021 CoQ
