MiPNet10.04 CellRespiration: Difference between revisions
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|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
|abstract=Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS | |||
Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; SchrΓΆcken, Austria). | |||
|info=[http://www.oroboros.at/index.php?id=protocols_cell_hrr_pc MiPNet10.04] | |info=[http://www.oroboros.at/index.php?id=protocols_cell_hrr_pc MiPNet10.04] | ||
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{{Labeling | {{Labeling | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|organism=Mouse | |organism=Mouse | ||
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|kinetics=Inhibitor; Uncoupler | |kinetics=Inhibitor; Uncoupler | ||
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential | |topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential | ||
|instruments=Oxygraph-2k, TIP2k, DatLab Software; Separate Application, Method | |||
|articletype=Protocol; Manual, MiPNet-online Publication | |||
}} | }} | ||
[[Category:OroboroPedia]] | [[Category:OroboroPedia]] |
Revision as of 11:44, 20 October 2010
Garedew A, Haffner B, HΓΌtter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr. Physiol. Network 10.04. |
Β» MiPNet10.04
Garedew A, Haffner B, HΓΌtter E, Gnaiger E (
)
Abstract: Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; SchrΓΆcken, Austria).
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Organism: Mouse
Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k, TIP2k, DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.