MiPNet19.05 IOC90 Bolzano IT

                

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MiPNet19.05 IOC90 Bolzano IT

Bolzano IT, 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. IOC90 at ISMM2014.

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OROBOROS (2014-05-27) Mitochondr Physiol Network

Abstract:

90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.

• O2k-Workshop
• http://www.ismm2014.org/GeneralInformation/Pages/News.aspx

• O2k-Network Lab: AT Innsbruck OROBOROS

Labels:

Stress:Ischemia-reperfusion, Temperature 

HRR: Oxygraph-2k 

ORO, IOC, 2014, MitoCom 

Conference

• ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31

O2k-Workshop

TUESDAY 2014-05-27 / 14.00 - 17.30

Seminar room 8

The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H₂O₂ production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.

» More details? Consult Hypothermia-Network: Mitochondria in the Cold.

Introduction

14:00 – 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments

Gnaiger Erich, CEO; Fontana-Ayoub Mona; Biomedical assistant; Fleischmann Sandra; Administrative assistant; Laner Verena; Project manager and scientific assistant

• OROBOROS INSTRUMENTS - General introduction into the Oxygraph-2k and high-resolution respirometry, O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module Titration-Injection microPump).

Demo experiment

14:45 – 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia

15:30 – 16:00 Coffee break

Round table: Hypoxia and mitochondrial function - practical experience

16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m

Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.

• Gnaiger Erich, AT - Boushel_RC, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK

Round table: Hypothermia and mitochondrial function - perspectives

16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function

Keynote statements by

• Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants

Participants

• Harald Engan (Mid Sweden University)
• Irene Pichler (EURAC Bolzano/Bozen - Center for Biomedicine)
• Lindsay Benage (Colorado State University)
• Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine)
• Mark Olfert (West Virginia University)
• Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin)
• Chris Müller (LMU München)
• Vittore Verrati (Chieti University)
• Marika Falla (Ospedale di Bolzano, Neurologia)
• Paolo Laveder (University of Padova, Italy, Dep of Biology)
• Daniel Martin (University College London)
• Edward Ojuka (University of Cape Town, South Africa)

Guest tutors and invited experts

• Robert Boushel (The Swedish School of Sport and Health Sciences)
• Adam Chicco (Colorado State University, Department of Health and Exercise Science)
• Andrew Murray (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
• James Horscroft (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
• Sandro Malacrida (EURAC Bolzano/Bozen - Center for Biomedicine)

Registration

• Visit us at the OROBOROS INSTRUMENTS exhibition booth from Monday to Wednesday, May 26 to 28.
• Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.

Recommended reading

• O2k-Publications: Hypoxia
• Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. »Abstract
• O2k-Publications: Oxygen kinetics
• O2k-Publications: Temperature

SUIT protocols for high-resolution respirometry

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access

• Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access

HRR and O2k-Fluorometry

» Manual: MiPNet17.05 O2k-Fluo LED2-Module

• Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access

» O2k-Fluorometry

Mitochondrial pathways

• Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. » Open Access - handout to O2k-Workshop participants.

O2k-Workshops are listed as MitoGlobal Events.

• This workshop is a component of the K-Regio project MitoCom.