MiPNet19.05 IOC90 Bolzano IT: Difference between revisions
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{{Publication | {{Publication | ||
|title=[[Image:OROBOROS-solo.png|right|50px|link= | |title=[[Image:OROBOROS-solo.png|right|50px|link=OROBOROS INSTRUMENTS]] | ||
'''Bolzano IT''', 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. '''IOC90''' at [http://www.ismm2014.org/Pages/default.aspx ISMM2014]. | '''Bolzano IT''', 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. '''IOC90''' at [http://www.ismm2014.org/Pages/default.aspx ISMM2014]. | ||
|info=[http://www.ismm2014.org/Programme/Pages/Applied-Workshop.aspx O2k-Workshop] | |info=[http://www.ismm2014.org/Programme/Pages/Applied-Workshop.aspx O2k-Workshop] | ||
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: [[Image:Gnaiger Erich.jpg|80px|link= | : [[Image:Gnaiger Erich.jpg|80px|link=Gnaiger_Erich|Erich Gnaiger]] [[Image:Mona.jpg|80px|link=Fontana-Ayoub Mona|Fontana-Ayoub Mona]] [[Image:FleischmannS.jpg|80px|link=Fleischmann Sandra|Fleischmann Sandra]] [[File:VG.jpg|80px|link=Laner Verena|Laner Verena]] | ||
<br /> | <br /> | ||
[[Gnaiger Erich]], CEO; | [[Gnaiger Erich]], CEO; | ||
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'''16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m''' | '''16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m''' | ||
: [[Image:Erich Gnaiger Wolstenhjolm Fjord March 2004.jpg|90px|link= | : [[Image:Erich Gnaiger Wolstenhjolm Fjord March 2004.jpg|90px|link=Gnaiger_Erich|Erich Gnaiger, Greenland]] [[Image:Boushel Robert C.JPG|100px|link=Boushel_Robert_C|Robert Boushel]] [[Image:Chicco Headshot.jpg|75px|link=Chicco_Adam_J|Adam Chicco]] [[Image:Murray Andrew J.jpg|130px|link=Murray_Andrew_J|Andrew Murray]] [[Image:Horscroft James A.jpg|85px|link=Horscroft_James_A|James Horscroft]] | ||
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function. | Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function. | ||
* [[Gnaiger Erich]], AT - [[Boushel Robert C]], SE - [[Chicco Adam J]], US - [[Murray Andrew J]], UK - [[Horscroft JA]], UK | * [[Gnaiger Erich]], AT - [[Boushel Robert C]], SE - [[Chicco Adam J]], US - [[Murray Andrew J]], UK - [[Horscroft JA]], UK | ||
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=== Participants === | === Participants === | ||
* Harald Engan (Mid Sweden University) | * Harald Engan (Mid Sweden University) | ||
* Irene Pichler (EURAC Bolzano/Bozen - Center for Biomedicine) | * [[Pichler I |Irene Pichler]] (EURAC Bolzano/Bozen - Center for Biomedicine) | ||
* Lindsay Benage (Colorado State University) | * [[Benage L |Lindsay Benage]] (Colorado State University) | ||
* Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine) | * Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine) | ||
* Mark Olfert (West Virginia University) | * Mark Olfert (West Virginia University) | ||
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* Marika Falla (Ospedale di Bolzano, Neurologia) | * Marika Falla (Ospedale di Bolzano, Neurologia) | ||
* Paolo Laveder (University of Padova, Italy, Dep of Biology) | * Paolo Laveder (University of Padova, Italy, Dep of Biology) | ||
* Daniel Martin (University College London) | * [[Martin D |Daniel Martin]] (University College London) | ||
* [[Ojuka E|Edward Ojuka]] (University of Cape Town, South Africa) | * [[Ojuka E|Edward Ojuka]] (University of Cape Town, South Africa) | ||
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* [[Murray Andrew J|Andrew Murray]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | * [[Murray Andrew J|Andrew Murray]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | ||
* [[Horscroft JA|James Horscroft]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | * [[Horscroft JA|James Horscroft]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | ||
* Sandro Malacrida (EURAC Bolzano/Bozen - Center for Biomedicine) | * [[Malacrida S|Sandro Malacrida]] (EURAC Bolzano/Bozen - Center for Biomedicine) | ||
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* [[O2k-Publications: Hypoxia]] | * [[O2k-Publications: Hypoxia]] | ||
:: Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3: 1135- | :: Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. [[Clanton 2013 Compr Physiol |»Abstract]] | ||
* [[O2k-Publications: Oxygen kinetics]] | * [[O2k-Publications: Oxygen kinetics]] | ||
* [[O2k-Publications: Temperature]] | * [[O2k-Publications: Temperature]] | ||
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=== SUIT protocols for high-resolution respirometry === | === SUIT protocols for high-resolution respirometry === | ||
[[Image:O2k-Publications.jpg|left|50px|link= | [[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] | ||
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol | | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »[[Pesta 2012 Methods Mol Biol |Bioblast Access]] | ||
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327- | * Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »[[Gnaiger_2008_POS |Bioblast Access]] | ||
=== HRR and O2k-Fluorometry === | === HRR and O2k-Fluorometry === | ||
* »[[MiPNet17.05 O2k-Fluorescence LED2-Module |Manual: O2k-Fluorescence LED2-Module]] | * »[[MiPNet17.05 O2k-Fluorescence LED2-Module |Manual: O2k-Fluorescence LED2-Module]] | ||
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. [[MiPNet18.05 Amplex-Mouse-heart | | * Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »[[MiPNet18.05 Amplex-Mouse-heart |Open Access]] | ||
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | * »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | ||
=== Mitochondrial pathways === | === Mitochondrial pathways === | ||
[[Image:O2k-Protocols.jpg|left|40px|link= | [[Image:O2k-Protocols.jpg|left|40px|link=O2k-Protocols|O2k-Protocols]] | ||
Gnaiger E ( | Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Open Access]] - '''''handout to O2k-Workshop participants'''''. | ||
[[Image:MitoGlobal.jpg|right|80px|link= | [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | ||
O2k-Workshops are listed as [[MitoGlobal Events]]. | O2k-Workshops are listed as [[MitoGlobal Events]]. | ||
* This workshop is a component of the K-Regio project ''[[MitoCom_K-Regio|MitoCom]]''. | * This workshop is a component of the K-Regio project ''[[MitoCom_K-Regio|MitoCom]]''. | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] |
Revision as of 06:51, 23 March 2015
Template:OROBOROS navigation line page name
Bolzano IT, 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. IOC90 at ISMM2014. |
OROBOROS (2014-05-27) Mitochondr Physiol Network
Abstract:
90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.
Info and preliminary programme: MiPNet19.05.pdf
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
Stress:Hypoxia, Temperature
HRR: Oxygraph-2k
ORO, IOC, 2014, MitoCom
Conference
- ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31
O2k-Workshop
- TUESDAY 2014-05-27 / 14.00 - 17.30
- Seminar room 8
The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.
- More details? Consult »Hypothermia-Network: Mitochondria in the Cold.
Introduction
14:00 – 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments
Gnaiger Erich, CEO;
Fontana-Ayoub Mona; Biomedical assistant;
Fleischmann Sandra; Administrative assistant;
Laner Verena; Project manager and scientific assistant
- OROBOROS INSTRUMENTS - General introduction into the Oxygraph-2k and high-resolution respirometry, O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module Titration-Injection microPump).
Demo experiment
14:45 – 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia
15:30 – 16:00 Coffee break
Round table: Hypoxia and mitochondrial function - practical experience
16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
- Gnaiger Erich, AT - Boushel Robert C, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK
Round table: Hypothermia and mitochondrial function - perspectives
16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function
Keynote statements by
- Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants
Participants
- Harald Engan (Mid Sweden University)
- Irene Pichler (EURAC Bolzano/Bozen - Center for Biomedicine)
- Lindsay Benage (Colorado State University)
- Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine)
- Mark Olfert (West Virginia University)
- Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin)
- Chris Müller (LMU München)
- Vittore Verrati (Chieti University)
- Marika Falla (Ospedale di Bolzano, Neurologia)
- Paolo Laveder (University of Padova, Italy, Dep of Biology)
- Daniel Martin (University College London)
- Edward Ojuka (University of Cape Town, South Africa)
Guest tutors and invited experts
- Robert Boushel (The Swedish School of Sport and Health Sciences)
- Adam Chicco (Colorado State University, Department of Health and Exercise Science)
- Andrew Murray (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
- James Horscroft (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
- Sandro Malacrida (EURAC Bolzano/Bozen - Center for Biomedicine)
Registration
- Visit us at the OROBOROS INSTRUMENTS exhibition booth from Monday to Wednesday, May 26 to 28.
- Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.
Recommended reading
- Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. »Abstract
SUIT protocols for high-resolution respirometry
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
HRR and O2k-Fluorometry
- »Manual: O2k-Fluorescence LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access
- »O2k-Fluorometry
Mitochondrial pathways
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.