MiPNet22.03 IOC119 Innsbruck AT: Difference between revisions

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{{OROBOROS header page name}}  
{{OROBOROS header page name}}  
{{Publication
{{Publication
|title= [[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]]
'''Innsbruck AT''', 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic '''IOC119'''.
'''Innsbruck AT''', 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic '''IOC119'''.
|info=
|info=
|authors=OROBOROS
|authors=Oroboros
|year=2017-02-27
|year=2017-02-27
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2017 Feb 28-Mar 03.
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2017 Feb 28-Mar 03.
|mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit
|mipnetlab=AT_Innsbruck_Oroboros , AT_Innsbruck_MitoFit
}}
}}
[[File:IOC119 Innsbruck.JPG|right|400px|Participants of IOC119 and the Oroboros tutors]]
__TOC__
__TOC__




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|
|
|-
|-
|15:30
|15:30-18:00
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|MitoFit ORO Lab
|ORO-MitoFit Lab
|-
|14:30-18:00
|Hands-on: Instrumental quality control 1 and 2.
|MitoFit ORO Lab - MiP''Art''
|-
|-
|}
|}
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|-
|-
|09:00
|09:00
|Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2
|Introduction to SUIT protocols RP1&RP2
|MiP''Art''
|MiP''Art''
|-
|-
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|15:30-18:00
|15:30-18:00
|Individual DatLab analysis + summary
|Individual DatLab analysis + summary
|MiP''Art''
|ORO-MitoFit Lab
|-
|-
|}
|}
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|14:00
|14:00
|DatLab analysis
|DatLab analysis
|MiP''Art''
|ORO-MitoFit Lab
|-
|-
|15:00
|15:00
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|15:30
|15:30
|DatLab analysis
|DatLab analysis
|MiP''Art''
|ORO-MitoFit Lab
|-
|-
|16:15
|16:15
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== General information ==
== General information ==
::::The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.  
::::The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.  
[[File:TRACT logo.png|right|120px|TRACT]]
::::Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.


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== Tutors ==
== Tutors ==
<br/>
<br/>
<gallery mode="nolines" widths="190px" heights="165px" perrow=6>
<gallery mode=default perrow=6 widths="140px" heights="150px">
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Gnaiger Erich]]'''
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Gnaiger Erich]]'''
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Sumbalova Zuzana]]'''
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Sumbalova Zuzana]]'''
File:NevesP.JPG|'''[[Neves P|Neves Pedro]]'''
File:NevesP.JPG|'''[[Neves P|Neves Pedro]]'''
File:PassruggerM.JPG|'''[[Passrugger M|Passrugger Manuela]]'''
File:PassruggerM.JPG|'''[[Passrugger M|Passrugger Manuela]]'''
File:GarciaL.JPG|'''[[Garcia e Souza LF|Garcia e Souza Luiz Felipe]]'''
File:GarciaL.JPG|'''[[Garcia-Souza LF|Garcia e Souza Luiz Felipe]]'''
File:Velika B.jpg|'''[[Velika B|Velika Beata]]'''
File:Velika B.jpg|'''[[Cizmarova B|Cizmarova Beata]]'''
</gallery>
</gallery>


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|  
|  
| [[Bufe A| Anja Bufe]]
| [[Bufe A| Anja Bufe]]
| '''[[AT Innsbruck OROBOROS]]:''' OROBOROS INSTRUMENTS, Innsbruck (AT)
| '''[[AT Innsbruck Oroboros ]]:''' Oroboros Instruments, Innsbruck (AT)
|-
|-
|  
|  
| [[Carvalho E| Eugenia Carvalho]]
| [[Carvalho E| Eugenia Carvalho]]
| '''[[US AR Little Rock Boersheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
| '''[[US AR Little Rock Borsheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
|-
|-
|  
|  
| [[Cotter M| Matthew Cotter]]
| [[Cotter M| Matthew Cotter]]
| '''[[US AR Little Rock Boersheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
| '''[[US AR Little Rock Borsheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
|-
|-
|  
|  
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| '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE)
| '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE)
|-
|-
| [[File:Guan YH.jpg|right|80px|Yue Hugh Guan]]
|  
| [[Guan YH| Yue Hugh Guan]]
| [[Guan YH| Yue Hugh Guan]]
| '''[[CN Shanghai Yi X]]:''' East China University of Science and Technology, Shanghai (CN)
| Shanghai (CN)
|-
|-
|  
|  
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|-
|-
|  
|  
| Anna-Maria Wintler
| [[Wintler AM|Anna-Maria Wintler]]
| '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE)
| '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE)
|-
|-
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:::: OROBOROS INSTRUMENTS
:::: Oroboros Instruments
:::: high-resolution respirometry  
:::: high-resolution respirometry  
:::: Schöpfstrasse 18
:::: Schöpfstrasse 18
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== Project support==
== Project support==
:::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
:::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
:::: [[File:Sponsors.png| 750 px]]
:::: [[File:Sponsors.png| 750 px]] [[File:TRACT logo.png|120px|TRACT]]


== IOC recommended reading ==
== IOC recommended reading ==
::::» '''[[IOC recommended reading]]'''
::::» '''[[IOC recommended reading]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]




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{{Labeling
{{Labeling
|additional=ORO, IOC, 2017, MitoFit, Next
|instruments=Oxygraph-2k
|additional=ORO, IOC, MitoFit, TRACT, 2017
}}
}}


[[Category:O2k-Workshops]]
[[Category:O2k-Workshops]]

Latest revision as of 10:56, 31 March 2021

                

MiPNet22.03 IOC119 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.


Oroboros (2017-02-27) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


Participants of IOC119 and the Oroboros tutors



Programme

Tuesday 28th Topic Room
17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 1st Topic Room
09:00 Mitochondrial and cell research MiPArt
10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt
10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt
12:00 Lunch break
13:00 Instrumental quality control 1: oxygen calibration MiPArt
14:00 Instrumental quality control 2: Instrumental O2 background MiPArt
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 2nd Topic Room
09:00 Introduction to SUIT protocols RP1&RP2 MiPArt
10:00 Coffee
10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt
12:00 Lunch break
13:00 Introduction to DatLab analysis MiPArt
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 3rd Topic Room
09:00 SUIT protocols MiPArt
10:30 Coffee
11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt
12:00 Lunch break
13:00 O2k-Core and O2k-Multisensor MiPArt
14:00 DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30 DatLab analysis ORO-MitoFit Lab
16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt
17:30 Farewell activity MiPArt

General information

The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
TRACT
Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).
» Information on travel and venue: IOC Innsbruck.

Registration

Fully booked.
Maximum number of 6 participants.

Tutors



Participants

Participant Institution
Anja Bufe AT Innsbruck Oroboros : Oroboros Instruments, Innsbruck (AT)
Eugenia Carvalho US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Matthew Cotter US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Ivone Leong SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Yue Hugh Guan Shanghai (CN)
Vasco Sequeira Oliveira NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL)
Anna-Maria Wintler SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Evelyn Zacharewicz NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)

Contact

[email protected]


Oroboros Instruments
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png TRACT

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


Labels:





HRR: Oxygraph-2k 

ORO, IOC, MitoFit, TRACT, 2017 

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