MiPNet23.03 IOC128 Cambridge UK: Difference between revisions
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| 09:15-10:15 | | 09:15-10:15 | ||
| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Lecture: '''Mitochondrial respiratory states and rates''' | Lecture: '''Mitochondrial respiratory states and rates. MitoEAGLE preprint''' | ||
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| 10:15-11:00 | | 10:15-11:00 | ||
| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Lecture: '''Mitochondrial preparations: the versatility of O2k applications''' Β | Lecture: '''Mitochondrial preparations: the versatility of O2k applications''' Β | ||
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| 11:15-12:15 | | 11:15-12:15 | ||
| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Demo experiment: '''Getting started with the O2k and DatLab 7''' | Demo experiment: '''Getting started with the O2k and DatLab 7''' | ||
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| 12:15-13:00 | | 12:15-13:00 | ||
| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Demo experiment: '''DatLab 7 innovations''' | Demo experiment: '''DatLab 7 innovations''' | ||
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| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Lecture: '''Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling | Lecture: '''Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling''' | ||
| [[Doerrier_2018_Methods_Mol_Biol]] | | [[Doerrier_2018_Methods_Mol_Biol]] | ||
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| 15:30-15:45 | | 15:30-15:45 | ||
| Tea break | | Tea break | ||
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| 15:45-17:00 | | 15:45-17:00 | ||
| [[ | | [[Meszaros A |Andras Meszaros]] | ||
Lecture: '''Normoxia β hyperoxia β hypoxia''' | |||
Lecture: '''Normoxia β hyperoxia β hypoxia | |||
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| 09:00-09:15 | | 09:00-09:15 | ||
|Arrival tea and coffee | |Arrival tea and coffee | ||
| Β | | Β | ||
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| 09:15-09:45 | | 09:15-09:45 | ||
| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Lecture: '''O2 flux and H2O2 production: physiology and pathology''' | Lecture: '''O2 flux and H2O2 production: physiology and pathology''' | ||
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Hands-on: '''O2k calibration and quality control (O2 and H2O2)''' Β | Hands-on: '''O2k calibration and quality control (O2 and H2O2)''' Β | ||
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| 11:00-11:15 | | 11:00-11:15 | ||
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| 11:15-13:00 | | 11:15-13:00 | ||
| Β | | Β | ||
Hands-on: '''O2k-FluoRespirometry for measurement of cell respiration and H2O2 production''' | Hands-on: '''O2k-FluoRespirometry for measurement of cell respiration and H2O2 production''' | ||
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| Lunch break | | Lunch break | ||
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| 14:00-15:30 | | 14:00-15:30 | ||
| | |Hands-on: '''Data analysis, interpretation and discussion''' | ||
Hands-on: '''Data analysis, interpretation and discussion | |||
| Β | | Β | ||
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| 15:30-15:45 | | 15:30-15:45 Β | ||
| Tea break | | Tea break | ||
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| 15:45-17:00 | | 15:45-17:00 | ||
| [[Gnaiger E |Erich Gnaiger]] | | [[Gnaiger E |Erich Gnaiger]] | ||
Lecture: '''O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence) | Lecture: '''O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence)''' | ||
| Β | | Β | ||
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Revision as of 20:35, 2 May 2018
MiPNet23.03 IOC128 Cambridge UK
Cambridge UK, 2018 May 7-8. Oroboros satellite O2k-Workshop on High-Resolution FluoRespirometry IOC128. |
Oroboros (2018-05-07) Mitochondr Physiol Network
Abstract:
Oroboros satellite O2k-Workshop on High-Resolution FluoRespirometry in cooperation with UK Cambridge Murray AJ. Cambridge, United Kingdom, 2018 May 7.
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS, UK Cambridge Murray AJ
General information
- This O2k-Workshop takes place satellite to the Mitochondrial Medicine Conference in Hinxton, UK. Separate registration is required for this workshop.
Venue
- May 7
- The Graham Storey Room
- Trinity Hall - Cambridge
- Cambridge CB2 1TJ
- United Kingdom
- Venue
- May 8
- Department of Physiology, Development & Neuroscience
- University of Cambridge
Local organizers
Registration
- Download registration form: download pdf
- Limited number of participants.
Tutors and Lecturers
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
AndrΓ‘s MΓ©szΓ‘ros, MD., PhD., Chief Research Officer, Oroboros Instruments
Verena Laner, Chief operating officer (COO), Oroboros Instruments
Programme
- May 7 - full day: lectures and presentations
- May 8 - morning: lectures and presentations
- May 8 - afternoon: hands-on session
- Topics:
- Mitochondrial respiratory states and rates
- SUIT Protocols in DatLab 7
- Mitochondrial preparations: the versatility of O2k applications
- Topics:
- Normoxia β hyperoxia β hypoxia
- O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence)
- O2 flux and H2O2 production: physiology and pathology
- Experimental demo for
- 1. Getting started with the O2k and DatLab 7
- 2. DatLab 7 innovations
- Experimental demo for
- Hands-on (2018-05-08)
- O2k calibration and quality control (O2 and H2O2)
- Cell respiration and H2O2 production
- Hands-on (2018-05-08)
Programme
Monday May 07
Time | Topic | Materials |
---|---|---|
09:00-09:15 | Arrival tea and coffee | |
09:15-10:15 | Erich Gnaiger
Lecture: Mitochondrial respiratory states and rates. MitoEAGLE preprint |
|
10:15-11:00 | Erich Gnaiger
Lecture: Mitochondrial preparations: the versatility of O2k applications |
|
11:00-11:15 | Morning coffee break | |
11:15-12:15 | Erich Gnaiger
Demo experiment: Getting started with the O2k and DatLab 7 |
|
12:15-13:00 | Erich Gnaiger
Demo experiment: DatLab 7 innovations |
|
13:00-14:00 | Lunch break | |
14:00-15:30 | Erich Gnaiger
Lecture: Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling |
Doerrier_2018_Methods_Mol_Biol |
15:30-15:45 | Tea break | |
15:45-17:00 | Andras Meszaros
Lecture: Normoxia β hyperoxia β hypoxia |
Tuesday May 08
Time | Topic | Materials |
---|---|---|
09:00-09:15 | Arrival tea and coffee | |
09:15-09:45 | Erich Gnaiger
Lecture: O2 flux and H2O2 production: physiology and pathology |
|
09:45-11:00 |
Hands-on: O2k calibration and quality control (O2 and H2O2) |
|
11:00-11:15 | Morning coffee break | |
11:15-13:00 |
Hands-on: O2k-FluoRespirometry for measurement of cell respiration and H2O2 production |
|
13:00-14:00 | Lunch break | |
14:00-15:30 | Hands-on: Data analysis, interpretation and discussion | |
15:30-15:45 | Tea break | |
15:45-17:00 | Erich Gnaiger
Lecture: O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence) |
Preliminary list of participants
Participant | Institution | |
---|---|---|
Davies Katie | University of Cambridge, UK | |
Fornaro Mara | Novartis Institutes for Biomedical Research, CH | |
Garrud Tessa | University of Cambridge, UK | |
Kapoor Rishab | Vanderbilt University Medical Center, USA | |
Knapp-Wilson Amber | University of Bristol , UK | |
Krause Fynn Nidas | University of Cambridge, UK | |
Martin Daniel | UK London Martin DS - University College London, UK | |
Sowton Alice | UK Cambridge Murray AJ - Trinity Hall, UK | |
Stanzani Giacomo | UK London Duchen MR - University College London, UK | |
Stevens Jia Liu | UK London Martin DS - University College London, UK | |
Tong Wen | University of Cambridge, UK | |
Yong Hannah | UK Cambridge Murray AJ - University of Cambridge, UK |
Mitochondrial Medicine Conference Hinxton
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- SchΓΆpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2018, Next
O2k-Workshops are listed as MitoGlobal Events.