MiPNet23.04 IOC133 Innsbruck AT

                

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MiPNet23.04 IOC133 Innsbruck AT

Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC.

Oroboros (2018-09-05) Mitochondr Physiol Network

Abstract:

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Sep 05-07.

• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit

General information

MitoFit Coaching Days - Basic provide a fundamental introduction to High-Resolution FluoRespirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.

Venue

» Information on travel and venue: IOC Innsbruck.

» Hotel suggestion: Hotel Weisses Kreuz

Registration

• closed

Tutors, lecturers, and managers

• 

  Carolina Doerrier, PhD., Oroboros Instruments - Chief scientific officer

• 

  Timea Komlòdi, PhD., Oroboros Instruments - Scientific researcher

• 

  Manuela Passrugger, BSc., Oroboros Instruments - Biomedical Assistant

• 

  Marco di Marcello, BSc., Medical University of Innsbruck, MitoFit project - Assistant

• 

  Erich Gnaiger, PhD., Oroboros Instruments, CEO

Programme

Tuesday 4th	Topic	Room
17:00 - 18:00	Welcome - common aims	MiPArt

Wednesday 5th	Topic	Room
09:00	Mitochondrial and cell research	MiPArt
10:00	OroboPOS service and O2k instrumental setup - overview with videoclips	MiPArt
10:30	Hands-on: OroboPOS service and O2k instrumental setup	MiPArt
12:00	Lunch break
13:00	Instrumental quality control 1: oxygen calibration	MiPArt
14:00	Instrumental quality control 2: Instrumental O2 background	MiPArt
15:00	Coffee
15:30-18:00	Hands-on: Instrumental quality control 1 and 2. DatLab analysis.	ORO-MitoFit Lab

Thursday 6th	Topic	Room
09:00	Introduction to SUIT protocols RP1&RP2	MiPArt
10:00	Coffee
10:30	O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.	MiPArt
12:00	Lunch break
13:00	Introduction to DatLab analysis	MiPArt
15:00	Coffee
15:30-18:00	Individual DatLab analysis + summary	ORO-MitoFit Lab

Friday 7th	Topic	Room
09:00	SUIT protocols	MiPArt
10:30	Coffee
11:00	Q&A session on HRR and OXPHOS analysis: Design of experimental protocols	MiPArt
12:00	Lunch break
13:00	O2k-Core and O2k-Multisensor	MiPArt
14:00	DatLab analysis	ORO-MitoFit Lab
15:00	Coffee
15:30	DatLab analysis	ORO-MitoFit Lab
16:15	The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions	MiPArt
17:30	Farewell activity	MiPArt

Participants

	Participant	Institution
	Bergmeister Lisa	AT_Innsbruck_Schwarzer C - Medical University, Innsbruck (AT)
	Daltro Cardoso Luiza Helena	AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
	Chowdhury Subir	CA Winnipeg Banerji V - University of Manitoba, Winnipeg (CA)
Garipi Enis	Garipi Enis	AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
	Huete-Ortega Maria	AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
	Neufang Nora Sophie	DE Hamburg Neufang NS - Universitätsklinikum Eppendorf, Hamburg (DE)
	Perez Valencia Juan Alberto	AT Innsbruck Oroboros - Leopold-Franzens-University, Innsbruck (AT)

Contact

[email protected]

Oroboros Instruments

High-Resolution FluoRespirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.

IOC recommended reading

» IOC recommended reading

» MitoPedia: Respiratory states

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

HRR: Oxygraph-2k 

ORO, IOC, MitoFit, SPIN, 2018, Next, MitoGlobal