Difference between revisions of "MiPNet28.06 IOC161 Coimbra PT"
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:::: '''Oroboros Instruments (Innsbruck, Austria) - 161<sup>st</sup> O2k-Workshop, Cantanhede''' | :::: '''Oroboros Instruments (Innsbruck, Austria) - 161<sup>st</sup> O2k-Workshop, Cantanhede''' | ||
:::: Cutting-edge innovations of '''high-resolution respirometry (HRR)''' are presented by ''Erich Gnaiger'', introducing the '''Oroboros NextGen-O2k''' [1] in parallel to hands-on training by ''Sabine Schmitt''. | :::: Cutting-edge innovations of '''high-resolution respirometry (HRR)''' are presented by ''Erich Gnaiger'', introducing the '''Oroboros NextGen-O2k''' [1] in parallel to hands-on training by ''Sabine Schmitt''. | ||
:::: HRR targets reproducibility and flexibility of '''substrate-uncoupler-inhibitor titration protocols''' [2] for investigating '''mitochondrial pathway and coupling control''' [3]. | :::: HRR targets reproducibility and flexibility of '''substrate-uncoupler-inhibitor titration protocols''' [2] for investigating '''mitochondrial pathway and coupling control''' [3]. ROS production, ATP production, mt-membrane potential, and calcium uptake can be measured simultaneously with HRR in each chamber. Modules for monitoring '''Q- and NADH-redox states and PhotoBiology''' with the NextGen-O2k open new windows for bioenergetic investigations. | ||
::::# [https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/ NextGen-O2k all-in-one] | ::::# [https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/ NextGen-O2k all-in-one] | ||
::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. | ::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. | ||
::::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | ::::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | ||
::::# Simultaneous measurement of mitochondrial respiration and [[hydrogen peroxide]] production ([[ROS]]) | |||
== Venue and organizers == | == Venue and organizers == | ||
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File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD, Oroboros Instruments - ''Mitochondrial Detective'' | File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD, Oroboros Instruments - ''Mitochondrial Detective'' | ||
</gallery> | </gallery> | ||
== Participants == | |||
<gallery mode=default perrow=7 widths="140px" heights="150px"> | |||
File:Profile-icon-9.png|'''[[Mota Sandra]]''', <br>University of Coimbra, PT | |||
File:Profile-icon-9.png|'''[[Anjo Sandra]]''', <br>University of Coimbra, PT | |||
File:Profile-icon-9.png|'''[[Pereira Susana P]]''', <br>University of Coimbra, PT | |||
|- | |||
</gallery> | |||
Latest revision as of 14:32, 19 April 2023
MiPNet28.06 IOC161 Coimbra PT
Cantanhede, PT, 2023 Apr 12. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC161. |
Oroboros (2023-04-12) Mitochondr Physiol Network
Abstract:
Oroboros O2k-Workshop on high-resolution respirometry (HRR) in cooperation with Oliveira Paulo J. Cantanhede, PT, 2023 April 12.
• O2k-Network Lab: AT_Innsbruck_Oroboros
General information
- Oroboros Instruments (Innsbruck, Austria) - 161st O2k-Workshop, Cantanhede
- Cutting-edge innovations of high-resolution respirometry (HRR) are presented by Erich Gnaiger, introducing the Oroboros NextGen-O2k [1] in parallel to hands-on training by Sabine Schmitt.
- HRR targets reproducibility and flexibility of substrate-uncoupler-inhibitor titration protocols [2] for investigating mitochondrial pathway and coupling control [3]. ROS production, ATP production, mt-membrane potential, and calcium uptake can be measured simultaneously with HRR in each chamber. Modules for monitoring Q- and NADH-redox states and PhotoBiology with the NextGen-O2k open new windows for bioenergetic investigations.
- NextGen-O2k all-in-one
- SUIT protocols - The SUITbrowser helps you find the best SUIT protocol for your specific research questions.
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- Simultaneous measurement of mitochondrial respiration and hydrogen peroxide production (ROS)
Venue and organizers
- UC Biotech Building, Lot 8A, Biocant Park
- 3060-197 Cantanhede
- Portugal
Dr. Oliveira Paulo J, University of Coimbra, PT
Erich Gnaiger, PhD, Oroboros Instruments - CEO, Innovation Alchemist
Sabine Schmitt, PhD, Oroboros Instruments - Mitochondrial Detective
Participants
Mota Sandra,
University of Coimbra, PTAnjo Sandra,
University of Coimbra, PTPereira Susana P,
University of Coimbra, PT
IOC recommended reading
O2k-Workshops are listed as MitoGlobal Events.'
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2023, MitoGlobal