MiRK03: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 15: | Line 15: | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
* For | === MiRK03 === | ||
{| class="wikitable" | |||
|- | |||
! substance !! MiRK03 !! molecular weight !! for 1 l | |||
|- | |||
| EGTA || 0.5 mM|| 380.35 g/mol || 190.18 mg | |||
|- | |||
| MgCl<sub>2</sub> x 6 H<sub>2</sub>O|| 3 mM|| 203.30 g/mol || 609.90 mg | |||
|- | |||
| HEPES || 20 mM|| 238.31 g/mol || 4 766.2 mg | |||
|- | |||
| KCl || 130 mM|| 74.56 g/mol || 9 692.8 mg | |||
|- | |||
| KH<sub>2</sub>PO<sub>4</sub>|| 10 mM|| 136.09 g/mol || 1 360.9 mg | |||
|- | |||
| BSA || 1 mg/ml || - || 1000 mg | |||
|} | |||
'''MiRK03 ph - temperature dependence''' | |||
data recorded with ''MiRK03 without BSA'' (only averaged values are shown; two measurements were conducted) | |||
{| class="wikitable" | |||
|- | |||
! temperature [°C] !! pH of MiRK03 | |||
|- | |||
| 20 || 7.15 | |||
|- | |||
| 25 || 7.12 | |||
|- | |||
| 30 || 7.09 | |||
|- | |||
| 35 || 7.05 | |||
|- | |||
| 37 || 7.03 | |||
|} | |||
* For comparison see: [[Mitochondrial respiration media: comparison]] |
Revision as of 18:17, 18 February 2016
Description
Mitochondrial respiration medium, MiRL03, modified after a medium described by Komary 2010 Biochim Biophys Acta, intended for use as medium for H2O2 production measurement with Amplex Red.
Abbreviation: MiRK03
Reference: Krumschnabel 2014 Methods Mol Biol, Komary 2010 Biochim Biophys Acta
MitoPedia topics: Media for respirometry
MiRK03
substance | MiRK03 | molecular weight | for 1 l |
---|---|---|---|
EGTA | 0.5 mM | 380.35 g/mol | 190.18 mg |
MgCl2 x 6 H2O | 3 mM | 203.30 g/mol | 609.90 mg |
HEPES | 20 mM | 238.31 g/mol | 4 766.2 mg |
KCl | 130 mM | 74.56 g/mol | 9 692.8 mg |
KH2PO4 | 10 mM | 136.09 g/mol | 1 360.9 mg |
BSA | 1 mg/ml | - | 1000 mg |
MiRK03 ph - temperature dependence
data recorded with MiRK03 without BSA (only averaged values are shown; two measurements were conducted)
temperature [°C] | pH of MiRK03 |
---|---|
20 | 7.15 |
25 | 7.12 |
30 | 7.09 |
35 | 7.05 |
37 | 7.03 |
- For comparison see: Mitochondrial respiration media: comparison