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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Karabatsiakis Alexander Austria Innsbruck Fischer Christine Austria Innsbruck Meszaros Andras T Austria Innsbruck Torres-Quesada Omar Austria Innsbruck Hoppel Florian Austria Innsbruck Gnaiger Erich Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Doerrier Carolina Belgium Leuven Bird Matthew Belgium Namur Arnould Thierry Belgium Liege Votion Dominique-Marie Belgium Namur Renard P Belgium Namur Detraux Damien Bosnia and Herzegovina Tuzla Jusic Amela Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil Sao Paulo Dominguez-Amorocho OA Brazil Sao Paulo Alencar Mayke Bezerra Brazil São Paulo Crispim Marcell Croatia Zagreb Rubelj Ivica Czech Republic Prague Fisar Zdenek Czech Republic Pilsen Kuncova J Czech Republic Prague 4 Bajzikova Martina Czech Republic Prague Pecina Petr Czech Republic Prague Hansikova Hana Czech Republic Prague Mracek Tomas Czech Republic Pilsen Kripnerova M Czech Republic Vestec Zobalova Renata Czech Republic Prague Elkalaf Moustafa Czech Republic Pilsen Markova Michaela Czech Republic Hradec Kralove Vernerova Andrea Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Copenhagen Desler C Denmark Copenhagen Rasmussen LJ Denmark Copenhagen N Mortensen OH Denmark Hvidovre Nehlin Jan O Denmark Aarhus Rattan Suresh IS Denmark Malov Hey-Mogensen M Egypt Cairo Abdel-Rahman Engy Ali Egypt Cairo Ali Sameh S Estonia Tallinn Truu Laura Estonia Tallinn Klepinina Lyudmila Estonia Tartu Grellier T Finland Helsinki Pirinen E Finland Jyvaeskylae Lehti Maarit Finland Turku Stier Antoine France Bordeaux cedex Mourier A France Bron Rizwan Alam M France Paris Petit Patrice X Germany Neuherberg Borchard Sabine Germany Ulm Kolassa Iris Germany Gießen Silaidos Carmina Germany Düsseldorf Prigione Alessandro Germany Freising Klingenspor Martin Germany Freising Fromme Tobias Germany Leipzig Ost Mario Germany Mainz Vujacic-Mirski Ksenija Germany Regensburg Renner-Sattler Kathrin Germany Duesseldorf Piel Sarah Germany Freising Keppner Gloria Germany Mainz Kalinovic S Germany Giessen Eckert Gunter P Germany Magdeburg Vieira Ligo Teixeira Camila Germany Munich Jastroch M Germany Biberach an der Riß Nold V Germany Hamburg Singer Dominique Germany Freising-Weihenstephan Musiol Eva Germany Ulm Hartmann C Germany Freising Schoettl T Germany Duesseldorf Eckers A Germany Ulm Calzia Enrico Germany Ulm Schaefer PM Greece Athens Trougakos Ioannis P Hungary Debrecen Verebne Tar K Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Ireland Dublin Karavyraki Marilena Ireland Dublin Porter Richard K Ireland Sligo Murphy J Israel Rishon Le Zion Hachmo Yafit Israel Jerusalem Saada Reisch Ann Israel Zrifin Sova Marina Italy Bologna Gasparre G Italy Rome Scatena Roberto Italy Milano Bottoni P Italy Verona Calabria Elisa Italy Bologna Genova Maria Luisa Italy Bolzano Pichler Irene Italy Bolzano/Bozen Volani Chiara Italy Bologna Paterlini S Italy Ancona Battino Maurizio Italy Bologna Porcelli AM Italy Padova Fernandez-Vizarra Erika Italy Bolzano Castelo Rueda Maria Paulina Japan Sapporo Shirakawa R Latvia Riga Makarova Elina Latvia Riga Kidere Dita Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Nijmegen Koopman Werner JH Netherlands Groningen Rots MG Netherlands Wageningen Keijer Jaap Norway Oslo Lund Jenny Norway Oslo Eide L Norway Oslo Nikolic N Norway Oslo Rustan AC Norway Bergen Tronstad Karl Johan Palestine Ramallah Stiban Johnny Poland Lodz Watala Cezary Poland Lodz Labieniec-Watala Magdalena Poland Poznań Ludziejewska A Poland Lodz Siewiera Karolina Poland Krakow Kaczara P Poland Poznan Michalak Slawomir Poland Poznan Swiniuch Daria Poland Warsaw Wasilewski M Poland Warsaw Kampa Rafal Pawel Poland Warsaw Dymkowska Dorota Poland Warsaw Sek Aleksandra Poland Poznan Rybacka-Mossakowska Joanna Portugal Cantanhede Pinho Sonia A Portugal Porto Beleza Jorge Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Porto Carrageta D Portugal Porto Magalhaes Jose Portugal Porto Martins Ana Dias Portugal Porto Crisostomo Luis Portugal Coimbra Carvalho Eugenia Portugal Porto Moreira Bruno P Portugal Cantanhede Bento Guida Jose Freitas Portugal Porto Oliveira Pedro Fontes Portugal Coimbra Palmeira Carlos Portugal Covilhã Dias Tania R Portugal Porto Bernardino RL Portugal Porto Alves Marco G Portugal Coimbra Pereira Susana P Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Korea Daejeon Park SH Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Lighezan R Romania Timisoara Betiu AM Romania Timisoara Avram Vlad F Romania Timisoara Danila Maria-Daniela Romania Timisoara Muntean Danina M Romania Timisoara Lelcu Theia Russia Moscow Itkis YS Russia Moscow Tsygankova P Russia Moscow Krylova T Saudi Arabia Giza Kotb IA Serbia Belgrad Markovic Ivanka Serbia Belgrade Misirlic-Dencic S Serbia Belgrade Jeremic M Serbia Belgrade Isakovic A Serbia Belgrade Ivanovic Tijana Serbia Belgrade Vukojevic M Slovakia Bratislava Sumbalova Zuzana Slovakia Košice Cizmarova Beata Slovakia Bratislava Ukropcova B Slovakia Bratislava Ukropec J Slovakia Bratislava Maderova D Slovakia Bratislava Nemec Michal Slovenia Ljubljana Pirkmajer Sergej Slovenia Ljubljana Mars T Slovenia Ljubljana Kopitar-Jerala Natasa Spain Granada Quiles JL Spain Barcelona Tura Ceide O Spain Sevilla Bustos Matilde Spain Salamanca Almeida Angeles Spain Barcelona Juarez D Spain Barcelona Arago Marc Spain Barcelona Zorzano Antonio Spain Barcelona Hyrossova Petra Spain A Coruña Mayan MD Spain Madrid De los Rios C Spain Barcelona Smolders V Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Barcelona Gil J Spain Barcelona Gama Perez Pau Spain Valencia Victor Victor Manuel Spain Armilla, Granada Escames Germaine Spain Madrid Cano-Abad MF Spain Ourense Reboredo-Rodriguez Patricia Spain Lleida Gonzalo Hugo Spain A Coruña Blanco FJ Spain Madrid Monsalve Maria Spain Madrid Lopez MG Spain Lleida Nogueras L Spain Valencia Borras Consuelo Spain Barcelona Dahdah Norma Ramon Spain Madrid Cadenas S Spain Valencia Fuertes Agudo Marina Spain Barcelona Gonzalez Casacuberta I Spain Sevilla Muntane Jordi Spain Valencia Casado Pinna Marta Sweden Linköping Fontes-Oliveira CC Sweden Lund Elmer Eskil Sweden Lund Chamkha Imen Sweden Södertälje Alfaro-Moreno E Sweden Lund Simon Serrano Sonia Sweden Lund Leffler M Sweden Lund Westerlund E Sweden Lund Abliva Sweden Lund Karlsson M Sweden, SE Lund Aasander Frostner Eleonor Switzerland Geneva Martinou JC Switzerland Lausanne Zanou Nadege The Netherlands BT Amsterdam Wuest Rob CI Turkey Tekirdag Aral Cenk Turkey Izmir Aydin HH Turkey Ankara Engin Ayse Basak Turkey Ankara Tuncay Erkan Turkey Istanbul Baykal AT USA Philadelphia Janowska Joanna USA Fayetteville Iyer Shilpa USA Iowa City Buettner Garry R USA St Louis Welch R USA Fayetteville Rao RR USA Philadelphia Kilbaugh Todd USA Orlando Coen Paul M USA Philadelphia Starr Jonathan USA Baton Rouge Irving Brian A United Kingdom Brighton Moore Anthony L United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Stoke-on-Trent McStay Gavin P United Kingdom Leicester Moisoi Nicoleta United Kingdom Birmingham Thakker A United Kingdom Birmingham Joanisse S Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
