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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Fischer Christine |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Torres-Quesada Omar |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Karabatsiakis Alexander |
Belgium | Namur | Detraux Damien |
Belgium | Leuven | Bird Matthew |
Belgium | Namur | Arnould Thierry |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Renard P |
Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
Bosnia and Herzegovina | Tuzla | Jusic Amela |
Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
Brazil | Sao Paulo | Dominguez-Amorocho OA |
Brazil | Sao Paulo | Alencar Mayke Bezerra |
Brazil | São Paulo | Crispim Marcell |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Prague | Pecina Petr |
Czech Republic | Prague 4 | Bajzikova Martina |
Czech Republic | Hradec Kralove | Vernerova Andrea |
Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
Czech Republic | Prague | Mracek Tomas |
Czech Republic | Vestec | Zobalova Renata |
Czech Republic | Prague | Hansikova Hana |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic | Pilsen | Kripnerova M |
Czech Republic | Prague | Fisar Zdenek |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Copenhagen | Rasmussen LJ |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Desler C |
Denmark | Malov | Hey-Mogensen M |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus | Rattan Suresh IS |
Egypt | Cairo | Ali Sameh S |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Estonia | Tallinn | Truu Laura |
Estonia | Tartu | Grellier T |
Estonia | Tallinn | Klepinina Lyudmila |
Finland | Helsinki | Pirinen E |
Finland | Jyvaeskylae | Lehti Maarit |
Finland | Turku | Stier Antoine |
France | Paris | Petit Patrice X |
France | Bron | Rizwan Alam M |
France | Bordeaux cedex | Mourier A |
Germany | Ulm | Hartmann C |
Germany | Freising | Klingenspor Martin |
Germany | Neuherberg | Borchard Sabine |
Germany | Leipzig | Ost Mario |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Duesseldorf | Piel Sarah |
Germany | Ulm | Kolassa Iris |
Germany | Freising | Keppner Gloria |
Germany | Gießen | Silaidos Carmina |
Germany | Düsseldorf | Prigione Alessandro |
Germany | Giessen | Eckert Gunter P |
Germany | Freising | Fromme Tobias |
Germany | Munich | Jastroch M |
Germany | Mainz | Vujacic-Mirski Ksenija |
Germany | Hamburg | Singer Dominique |
Germany | Biberach an der Riß | Nold V |
Germany | Duesseldorf | Eckers A |
Germany | Freising | Schoettl T |
Germany | Mainz | Kalinovic S |
Germany | Ulm | Calzia Enrico |
Germany | Magdeburg | Vieira Ligo Teixeira Camila |
Germany | Ulm | Schaefer PM |
Germany | Freising-Weihenstephan | Musiol Eva |
Greece | Athens | Trougakos Ioannis P |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Komlodi Timea |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Ireland | Dublin | Karavyraki Marilena |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Zrifin | Sova Marina |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Verona | Calabria Elisa |
Italy | Bolzano | Castelo Rueda Maria Paulina |
Italy | Bologna | Genova Maria Luisa |
Italy | Bolzano | Pichler Irene |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Bologna | Paterlini S |
Italy | Bologna | Porcelli AM |
Italy | Ancona | Battino Maurizio |
Italy | Bologna | Gasparre G |
Italy | Rome | Scatena Roberto |
Italy | Milano | Bottoni P |
Japan | Sapporo | Shirakawa R |
Latvia | Riga | Makarova Elina |
Latvia | Riga | Kidere Dita |
Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
Malta | Msida | Vella Joanna |
Netherlands | Nijmegen | Koopman Werner JH |
Netherlands | Wageningen | Keijer Jaap |
Netherlands | Groningen | Rots MG |
Norway | Oslo | Eide L |
Norway | Oslo | Lund Jenny |
Norway | Oslo | Nikolic N |
Norway | Oslo | Rustan AC |
Norway | Bergen | Tronstad Karl Johan |
Palestine | Ramallah | Stiban Johnny |
Poland | Poznan | Rybacka-Mossakowska Joanna |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Lodz | Siewiera Karolina |
Poland | Poznan | Michalak Slawomir |
Poland | Warsaw | Wasilewski M |
Poland | Krakow | Kaczara P |
Poland | Poznań | Ludziejewska A |
Poland | Warsaw | Dymkowska Dorota |
Poland | Poznan | Swiniuch Daria |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Lodz | Watala Cezary |
Poland | Warsaw | Sek Aleksandra |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Coimbra | Pereira Susana P |
Portugal | Porto | Bernardino RL |
Portugal | Porto | Alves Marco G |
Portugal | Cantanhede | Pinho Sonia A |
Portugal | Porto | Beleza Jorge |
Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
Portugal | Porto | Carrageta D |
Portugal | Porto | Magalhaes Jose |
Portugal | Porto | Martins Ana Dias |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Cantanhede | Bento Guida Jose Freitas |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Covilhã | Dias Tania R |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Daejeon | Park SH |
Republic of Serbia | Belgrade | Pavlovic Kasja |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Romania | Timisoara | Lelcu Theia |
Romania | Timisoara | Betiu AM |
Romania | Timisoara | Avram Vlad F |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Danila Maria-Daniela |
Romania | Timisoara | Lighezan R |
Russia | Moscow | Itkis YS |
Russia | Moscow | Tsygankova P |
Russia | Moscow | Krylova T |
Saudi Arabia | Giza | Kotb IA |
Serbia | Belgrade | Vukojevic M |
Serbia | Belgrad | Markovic Ivanka |
Serbia | Belgrade | Misirlic-Dencic S |
Serbia | Belgrade | Ivanovic Tijana |
Serbia | Belgrade | Jeremic M |
Serbia | Belgrade | Isakovic A |
Slovakia | Bratislava | Nemec Michal |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Ukropec J |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Bratislava | Maderova D |
Slovenia | Ljubljana | Mars T |
Slovenia | Ljubljana | Kopitar-Jerala Natasa |
Slovenia | Ljubljana | Pirkmajer Sergej |
Spain | Valencia | Fuertes Agudo Marina |
Spain | Madrid | De los Rios C |
Spain | Barcelona | Gama Perez Pau |
Spain | Sevilla | Muntane Jordi |
Spain | Armilla, Granada | Escames Germaine |
Spain | Sevilla | Bustos Matilde |
Spain | Salamanca | Almeida Angeles |
Spain | Madrid | Cano-Abad MF |
Spain | Barcelona | Tura Ceide O |
Spain | A Coruña | Blanco FJ |
Spain | Madrid | Monsalve Maria |
Spain | Barcelona | Hyrossova Petra |
Spain | Barcelona | Arago Marc |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Madrid | Lopez MG |
Spain | Barcelona | Smolders V |
Spain | Barcelona | Gil J |
Spain | Valencia | Victor Victor Manuel |
Spain | Madrid | Cadenas S |
Spain | Ourense | Reboredo-Rodriguez Patricia |
Spain | Lleida | Gonzalo Hugo |
Spain | Barcelona | Gonzalez Casacuberta I |
Spain | Valencia | Casado Pinna Marta |
Spain | Valencia | Borras Consuelo |
Spain | Granada | Quiles JL |
Spain | Lleida | Nogueras L |
Spain | Barcelona | Juarez D |
Spain | Barcelona | Dahdah Norma Ramon |
Spain | Barcelona | Zorzano Antonio |
Spain | A Coruña | Mayan MD |
Sweden | Lund | Chamkha Imen |
Sweden | Södertälje | Alfaro-Moreno E |
Sweden | Lund | Westerlund E |
Sweden | Lund | Simon Serrano Sonia |
Sweden | Lund | Karlsson M |
Sweden | Lund | Leffler M |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden | Lund | Elmer Eskil |
Sweden | Lund | Abliva |
Sweden, SE | Lund | Aasander Frostner Eleonor |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Geneva | Martinou JC |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Izmir | Aydin HH |
Turkey | Ankara | Engin Ayse Basak |
Turkey | Ankara | Tuncay Erkan |
Turkey | Istanbul | Baykal AT |
Turkey | Tekirdag | Aral Cenk |
USA | Philadelphia | Starr Jonathan |
USA | Philadelphia | Janowska Joanna |
USA | St Louis | Welch R |
USA | Fayetteville | Rao RR |
USA | Iowa City | Buettner Garry R |
USA | Philadelphia | Kilbaugh Todd |
USA | Baton Rouge | Irving Brian A |
USA | Orlando | Coen Paul M |
USA | Fayetteville | Iyer Shilpa |
United Kingdom | Brighton | Moore Anthony L |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Leicester | Moisoi Nicoleta |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Stoke-on-Trent | McStay Gavin P |
United Kingdom | Birmingham | Joanisse S |
Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).