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Difference between revisions of "MitoPediaAlert2020 Archive"

From Bioblast
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|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«<br>
- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''« <br>
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br>
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« <br>
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
|}
|}
<br />
</div>
 
</div>
== Apr 2020 ==
== Apr 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]
[[File:Expand.png|right|35px |Click to expand or collaps]]
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].<br>
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]]. <br>
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
|}
|}
<br />
<br>
* '''Apr-21 Tu'''  
* '''Apr-21 Tu'''  
[[File:CS.png|right|100px|Citrate synthase]]
[[File:CS.png|right|100px|Citrate synthase]]
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.<br>
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. <br>
- »'''[[MiPNet17.04 CitrateSynthase]]'''«
- »'''[[MiPNet17.04 CitrateSynthase]]'''«
|}
|}
<br />
<br>
* '''Apr-13 Mo'''
* '''Apr-13 Mo'''
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?'''<br>
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br>
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]]<br>
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br>
- »'''[[SUIT-024 |SUIT-024]]'''«
- »'''[[SUIT-024 |SUIT-024]]'''«
|}
|}
<br />
<br>
* '''Apr-08 We'''
* '''Apr-08 We'''
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''<br>
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' <br>
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration.<br>
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. <br>
- »'''[[SUIT-010 |SUIT-010]]'''«
- »'''[[SUIT-010 |SUIT-010]]'''«
|}
|}
<br />
<br>
* '''Apr-02 Th'''  
* '''Apr-02 Th'''  
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]  
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]  
|'''Do you want to search the best [[SUIT]] protocol for your research questions?'''<br>
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' <br>
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«
|}
|}
<br />
</div>
 
</div>
== Mar 2020 ==
== Mar 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]
[[File:Expand.png|right|35px |Click to expand or collaps]]
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:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| '''The standardized coupling control protocol to explore the bioenergetics of living cells'''<br>
| '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br>
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.<br>
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. <br>
- »'''[[SUIT-003 |SUIT-003]]'''«
- »'''[[SUIT-003 |SUIT-003]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]].<br>
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. <br>
- »'''[[Blebbistatin]]'''«
- »'''[[Blebbistatin]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols.<br>
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br>
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]?<br>
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? <br>
- »'''[[Rapamycin]]'''«
- »'''[[Rapamycin]]'''«
|}
|}
 
</div>
</div>
== Feb 2020 ==
== Feb 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]
[[File:Expand.png|right|35px |Click to expand or collaps]]
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[electron transfer capacity]].
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[electron transfer capacity]]. <br>
- »'''[[Noncoupled respiration]]'''«
- »'''[[Noncoupled respiration]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| More research is needed on the impact of viral infection on mitochondrial function.
| More research is needed on the impact of viral infection on mitochondrial function. <br>
- »'''[[Viruses and mitochondrial medicine]]'''«
- »'''[[Viruses and mitochondrial medicine]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. <br>
- »'''[[Spermidine]]'''«
- »'''[[Spermidine]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| Why do we fight gas bubbles?
| Why do we fight gas bubbles? <br>
- Dive deep into »'''[[Oxygen solubility]]'''«
- Dive deep into »'''[[Oxygen solubility]]'''«
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| What do metformin, melatonin and other bioactive mitObesity compounds have in common?
| What do metformin, melatonin and other bioactive mitObesity compounds have in common? <br>
- »'''[[MitObesity drugs]]'''«
- »'''[[MitObesity drugs]]'''«
|}
|}
 
</div>
</div>
== Jan 2020 ==
== Jan 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]
[[File:Expand.png|right|35px |Click to expand or collaps]]
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| '''[[Oxygen flux]]'''
| '''[[Oxygen flux]]''' <br>
- which SI units should be used?
- which SI units should be used?
|}
|}
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:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| Did you ever think that units for body mass could be an issue?
| Did you ever think that units for body mass could be an issue? <br>
- »'''[[Body mass]]'''«
- »'''[[Body mass]]'''«
|}
|}
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| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.
| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.
|}
|}
</div>
</div>

Revision as of 10:03, 5 May 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

  • May-07 Th
MitoPedia: SUIT Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?

Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate anaplerotic pathway control state« 
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027« 
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«

Apr 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

  • Apr-24 Fr
Electron transport chain
MitoPedia ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«


  • Apr-21 Tu
Citrate synthase
MitoPedia Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«


  • Apr-13 Mo
MitoPedia: SUIT Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?

DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«


  • Apr-08 We
MitoPedia: SUIT The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.

DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«


  • Apr-02 Th
MitoPedia: SUIT Do you want to search the best SUIT protocol for your research questions?

- Use the SUITbrowser: »SUITbrowser«

Mar 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

  • Mar-16 Mo
MitoPedia: SUIT The standardized coupling control protocol to explore the bioenergetics of living cells

DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- »SUIT-003«


  • Mar-11 We
MitoPedia Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«


  • Mar-06 Fr
MitoPedia: SUIT Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«


  • Mar-03 Tu
MitoPedia Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«

Feb 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

  • Feb-26 We
MitoPedia It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«


  • Feb-24 Mo
MitoPedia More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«


  • Feb-19 We
MitoPedia Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«


  • Feb-18 Tu
MitoPedia Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«


  • Feb-12 We
MitoPedia What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

  • Jan-16 Th
MitoPedia Oxygen flux

- which SI units should be used?


MitoPedia Did you ever think that units for body mass could be an issue?

- »Body mass«


  • Jan-08 We
MitoPedia Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.
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