Difference between revisions of "MitoPediaAlert2020 Archive"
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Revision as of 08:18, 20 July 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2020
- Jul-23 Th
Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations? It is possible to do so using exogenously added cytochrome c:
- »Cytochrome c control factor«
- Jul-16 Th
Do you know how to select the proper Fluorescence-Sensor and filter set?
Using different Fluorescence-Sensors and Filters, the O2k-Fluo Smart-Module allows for measurement of Hydrogen peroxide, ATP, Mitochondrial membrane potential, Calcium, and numerous other applications open for O2k-user innovation.
- » Selecting the Smart Fluo-Sensor« and » Setup of the O2k-Fluo LED2-Module«
- Jul-09 Th
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«