O2k-OpenSupportAlert2020 Archive: Difference between revisions
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| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png| | | width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]] | ||
|Do you know how to | |'''Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.''' <br> | ||
- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. | |||
- Learn more about - | - ยป[[Steady state]]ยซ | ||
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* '''May-04 Mo''' | |||
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| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]] | |||
|'''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?''' | |||
- See how to perform the instrumental background test. | |||
- ยป[[Flux / Slope| O2 background correction in DatLab]]ยซ and ยป[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]ยซ | |||
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== Apr 2020 == | == Apr 2020 == |
Revision as of 09:58, 4 May 2020
- Currently Answered Questions from the ยปOroboros Ecosystem Agendaยซ
May 2020
- May-11 Th
Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - ยปSteady stateยซ
- May-04 Mo
Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels? - See how to perform the instrumental background test. - ยป O2 background correction in DatLabยซ and ยป Instrumental oxygen backgroundยซ
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases? - DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - ยปSUIT-024ยซ
- Apr-08 We
The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage. - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - ยปSUIT-010ยซ
- Apr-02 Th
Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: ยปSUITbrowserยซ
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - ยปSUIT-003ยซ
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - ยปBlebbistatinยซ
- Mar-06 Fr
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
Rapamycin - more differences or more in common with other mitObesity drugs? - ยปRapamycinยซ
Feb 2020
- Feb-26 We
It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity. - ยปNoncoupled respirationยซ
- Feb-24 Mo
- Feb-19 We
Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - ยปSpermidineยซ
- Feb-18 Tu
Why do we fight gas bubbles? - Dive deep into ยปOxygen solubilityยซ
- Feb-12 We
What do metformin, melatonin and other bioactive mitObesity compounds have in common? - ยปMitObesity drugsยซ
Jan 2020
- Jan-16 Th
Oxygen flux - which SI units should be used?
Did you ever think that units for body mass could be an issue? - ยปBody massยซ
- Jan-08 We
Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.