O2k-OpenSupportAlert2020 Archive: Difference between revisions
No edit summary |
|||
Line 3: | Line 3: | ||
== Jul 2020 == | == Jul 2020 == | ||
2020 Week 31 | |||
* '''Jul-27 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | |||
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?''' | |||
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br> | |||
- » [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]« | |||
|} | |||
---- | |||
* '''Jul-20 Mo''' | * '''Jul-20 Mo''' | ||
:{| | :{| |
Revision as of 08:10, 27 July 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2020
2020 Week 31
- Jul-27 Mo
Do you know how to prepare a homogenate from tissue samples using the PBI-Shredder O2k-Set? The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality.
- » O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation«
- Jul-20 Mo
How can you compare respirometric experiments using an internal normalization?
Flux control ratios (FCR) are independent of external measurements (e.g. protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”.
- »Flux control ratio«Read more: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«
- Jul-13 Mo
Did you know that you can apply instrumental O2 background test parameters (i.e., intercept and slope) from an existing file in DatLab?
In the DatLab file, you can copy the Instrumental O2 background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. For more information, see: - »Copy from file«
- Jul-06 Mo
Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature?
During air calibration, temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the temperature control, an error message will appear. For more information, see: - »Block temperature does not increase while heating with full power« and »DatLab error messages «