O2k-OpenSupportAlert2020 Archive
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2020
- Jul-20 Mo
How can you compare respirometric experiments using an internal normalization?
Flux control ratios (FCR) are independent of external measurements (e.g. protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”.
- »Flux control ratio«Read more: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«
- Jul-13 Mo
Did you know that you can apply instrumental O2 background test parameters (i.e., intercept and slope) from an existing file in DatLab?
In the DatLab file, you can copy the Instrumental O2 background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. For more information, see: - »Copy from file«
- Jul-06 Mo
Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature?
During air calibration, temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the temperature control, an error message will appear. For more information, see: - »Block temperature does not increase while heating with full power« and »DatLab error messages «