From Bioblast
O2k-Open Support alert |
We provide service and support based on open innovation
- - listed in the Oroboros Ecosystem Agenda 2020
Mar 2020
- Mar-04 We
- Do you know that a damaged OroboPOS-Holder might lead to a leaky chamber?
- After chamber reassembly you might experience a leaky chamber due to damaged O2k-parts, specially the OroboPOS-Holder. - ยป Leaky chamberยซ
- Do you know that a damaged OroboPOS-Holder might lead to a leaky chamber?
- Mar-11 We
- Mar-18 We
- Do you change the slope smoothing to 20 while running experiments?
- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - ยปSlope smoothingยซ
- Do you change the slope smoothing to 20 while running experiments?
Feb 2020
- Feb-28 Fr
- Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - ยป Oxygen calibration - DatLabยซ
- Calibration parameters are transferred to experimental DatLab files
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - ยปOxygen calibration - DatLabยซ
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - ยปOxygen calibration - DatLabยซ
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark โR1โ and click on Calibrate and copy to clipboard in the O2 calibration window. - ยปOxygen calibration - DatLabยซ
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the โO2k configurationโ window?
- Including the POS serial number in the ยดO2k configurationยด window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. ยปO2k configurationยซ
- Do you write the serial number of the polarographic oxygen sensor (POS) in the โO2k configurationโ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - ยปO2k-Chamber MitoPediaยซ
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the โO2 calibrationโ window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. - ยปOxygen solubility factorยซ
- Do you use the correct O2 solubility factor (FM) in the โO2 calibrationโ window in DatLab?
Stay alert
Collections from Bioblast agenda