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O2k-Publications: O2k-Protocol
Sort in ascending/descending order by a click on one of the small symbols in squares below. Default sorting: chronological. Empty fields appear first in ascending order.
Year | Reference | Organism | Tissue;cell | |
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Baglivo 2022 BEC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioenerg Commun 2022.8. https://doi.org/10.26124/bec:2022-0008 | HEK | |
Baglivo 2022 MitoFit-QC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 â 2022-08-04 published in Bioenerg Commun 2022.8. | HEK | |
MiPNet24.10 H2O2 flux analysis | 2021-10-22 | Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4 | ||
MiPNet26.10 MgG data analysis | 2021-09-16 | Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4 | ||
MiPNet24.09 Data analysis of mt-membrane potential | 2021-09-16 | Data analysis of mt-membrane potential. | ||
MiPNet17.05 O2k-Fluo LED2-Module | 2021-06-22 | O2k-Fluo LED2-Module. | ||
Went 2021 MitoFit PB | 2021 | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005 | Algae | |
Komlodi 2021 BEC Q | 2021 | KomlĂłdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003 | Mouse | Heart Nervous system |
Cardoso 2021 BEC MgG | 2021 | Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001 | Mouse | Heart |
Komlodi 2021 BEC AmR-O2 | 2021 | KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004 | Saccharomyces cerevisiae | Other cell lines |
MiPNet21.17 BloodCellsIsolation | 2020-10-22 | O2k-Protocols: Isolation of blood cells for HRR. | Human | Blood cells Platelet |
MiPNet24.08 Safranin Analysis Template | 2020-05-13 | Excel template for safranin data analysis. | ||
MiPNet17.04 CitrateSynthase | 2020-04-18 | Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. | ||
MiPNet24.06 Oxygen flux analysis - DatLab 7.4 | 2020-04-16 | Oxygen flux analysis with DatLab 7.4. | ||
MiPNet25.14 TPP Analysis Template | 2020-##-## | Excel template for TPP data analysis. | ||
Gnaiger 2020 BEC MitoPathways | 2020 | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | Human Mouse | Heart Skeletal muscle Fibroblast |
MiPNet21.16 DatLab 7 Innovations | 2019-09-23 | DatLab 7: innovations. | ||
MiPNet23.12 DL-Protocols help | 2019-09-13 | DatLab Protocols: Help DatLab 7. | ||
MiPNet22.16 DatLab 7 Innovations: DL-Protocols | 2019-09-13 | DatLab 7.4 Innovations: DL-Protocols | ||
MiPNet24.11 mtMP calculation | 2019-08-05 | Mitochondrial membrane potential calculation | ||
MiPNet20.13 Safranin mt-membranepotential | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. | Mouse | Nervous system |
MiPNet18.05 Amplex-Mouse-heart | 2019-06-24 | O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. | Mouse | Heart |
MiPNet20.14 AmplexRed H2O2-production | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed. | Mouse | Heart |
Long 2019 Methods Mol Biol | 2019 | Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46. | Mouse | Heart |
Naszai 2019 Sci Rep | 2019 | NĂĄszai A, Terhes E, Kaszaki J, Boros M, JuhĂĄsz L (2019) Ca(2+)N it be measured? Detection of extramitochondrial calcium movement with high-resolution FluoRespirometry. Sci Rep 9:19229. | Rat | Liver |
MiPNet12.01 Suppl T-issue | 2018-11-18 | MitoPathways at the Q-junction: mouse skeletal muscle fibers. | Mouse | Skeletal muscle |
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MĂ©szĂĄros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Komlodi 2018 Methods Mol Biol | 2018 | Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. | Human Mouse | Skeletal muscle HEK |
Lemieux 2017 Sci Rep | 2017 | Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8 | Mouse | Heart |
MiPNet21.14 Reference sample HRR | 2016-10-19 | Development of a reference sample for HRR. | Human | HEK |
MiPNet21.12 Comparison mt respiration media | 2016-10-18 | O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry. | Human | HEK |
MiPNet21.13 Comparison mt-preparations | 2016-10-18 | O2k-Protocols: Mitochondrial preparations for HRR. | Mouse | Heart |
MiPNet08.09 CellRespiration | 2016-08-11 | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. | Human | Blood cells Lymphocyte |
MiPNet19.18E O2 flux analysis - DatLab 6 | 2016-08-08 | Oxygen flux analysis: DatLab real-time. | ||
MiPNet10.04 CellRespiration | 2016-08-08 | An experiment with high-resolution respirometry: coupling control in cell respiration. | Mouse | Blood cells |
MiPNet21.07 MitoFit DatLab PT | 2016-07-20 | MitoFit DatLab proficiency test. | Human | |
MiPNet17.03 Shredder vs Fibers | 2015-06-18 | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. | Fishes | Heart Liver |
Gnaiger 2015 Scand J Med Sci Sports | 2015 | Gnaiger E, Boushel R, SĂžndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DĂez-SĂĄnchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612 | Human | Skeletal muscle |
Krumschnabel 2015 Methods Mol Biol | 2015 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. | Mouse | Nervous system |
Makrecka-Kuka 2015 Biomolecules | 2015 | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 | Human Mouse | Heart Nervous system HEK |
MiPNet12.11 MitoRespiration | 2014-02-20 | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. | Artemia Crustaceans | |
Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Mouse | Nervous system |
Van Bergen 2014 Mitochondrion | 2014 | Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33. | Human Mouse | Nervous system Blood cells |
Chinopoulos 2014 Methods Enzymol | 2014 | Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. doi:10.1016/B978-0-12-416618-9.00017-0 | Human | HEK |
Elustondo 2014 Biochim Biophys Acta | 2014 | Elustondo PA, Negoda A, Kane CL, Kane DA, Pavlov EV (2014) Spermine selectively inhibits high-conductance, but not low-conductance calcium-induced permeability transition pore. Biochim Biophys Acta 1847:231â40. | Rat | Liver |
Pesta 2012 Methods Mol Biol | 2012 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Human | Skeletal muscle Other cell lines HEK Fibroblast HUVEC |
MiPNet08.12 IOC22 | 2011-12-11 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. | Rat | Liver |
Lemieux 2011 Int J Biochem Cell Biol | 2011 | Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729â38. https://doi.org/10.1016/j.biocel.2011.08.008 | Human | Heart |
Aguirre 2010 Biochim Biophys Acta | 2010 | Aguirre E, RodrĂguez-JuĂĄrez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. https://doi.org/10.1016/j.bbabio.2010.01.033 | HEK | |
Gnaiger 2009 Int J Biochem Cell Biol | 2009 | Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837-45. https://doi.org/10.1016/j.biocel.2009.03.013 | Human Mouse | Skeletal muscle |
Gnaiger 2008 POS | 2008 | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | Mouse | Blood cells |
Gnaiger 2007 MitoPathways | 2007 | Gnaiger E ed (2007) Mitochondrial pathways and respiratory control. 1st ed. Oroboros MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-0-4. â see 5th edition: Gnaiger 2020 BEC MitoPathways. | ||
Huetter 2004 Biochem J | 2004 | HĂŒtter E, Renner K, Pfister G, Stöckl P, Jansen-DĂŒrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. https://doi.org/10.1042/BJ20040095 | Human | Fibroblast |
Gnaiger 2000 Life in the Cold | 2000 | Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Berlin, Heidelberg:431-42. https://doi.org/10.1007/978-3-662-04162-8_45 | Rat | Heart |
MiPNet04.05 Titration-Injection | 1999-01 | From step titration to ramp injection: Uncoupling by FCCP with TIP. | Human | Endothelial;epithelial;mesothelial cell HUVEC |
Steinlechner-Maran 1996 Am J Physiol Cell Physiol | 1996 | Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. https://doi.org/10.1152/ajpcell.1996.271.6.C2053 | Human | Endothelial;epithelial;mesothelial cell HUVEC |
O2k-Publications: O2k-Protocol- Abstracts
Sort in ascending/descending order by a click on one of the small symbols in squares below.
Year | Reference | Organism | Tissue;cell | |
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Bastos Sant'Anna Silva 2019 Life Sciences Meeting 2019 Innsbruck AT | 2019 | Cellular succinate transport and mitochondrial respiratory function in prostate cancer. | Human | Genital |
Garcia-Roves 2019 MiPschool Coimbra | 2019 | Generating reference values on mitochondrial respiration in permeabilized muscle fibers. | Mouse | Skeletal muscle |
Calabria 2019 MiPschool Coimbra | 2019 | Investigating mitochondrial function in blood cells by high-resolution respirometry: contribution to an interlaboratory study. MitoEAGLE WG4. | Blood cells | |
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT | 2018 | pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells â a pilot study. | Human | HEK Kidney |
Sobotka 2018 MiP2018 | 2018 | Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay. | Mouse Saccharomyces cerevisiae | Heart Nervous system |
Doerrier 2018 ESCI2018 | 2018 | High-resolution respirometry, two techniques combined in one to explore mitochondrial function in health and disease | Human Mouse | Heart Skeletal muscle Nervous system Blood cells HEK |
Bastos Sant'Anna Silva 2018 TRANSMIT Bertinoro di Romagna IT | 2018 | Cellular succinate transport and mitochondrial respiratory function in prostate cancer. | Human | HEK Genital |
Bastos Sant'Anna Silva 2018 MiPschool Tromso C4 | 2018 | Role of succinate in prostate cancer cells: uptake and mitochondrial respiratory function. | Human | Genital |
Garipi 2018 MiPschool Tromso E2 | 2018 | Phenotyping mitochondrial metabolism in Barrettâs metaplasia-dysplasia-adenocarcinoma sequence: respiratory capacity, extracelular proton flux and ROS production | Human | Endothelial;epithelial;mesothelial cell Other cell lines |
Sobotka 2017 MiPschool Obergurgl | 2017 | Reference protocols for ROS production measurements. | ||
Doerrier 2016 Abstract MitoFit Science Camp 2016 | 2016 | Development of a SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. | Human Mouse | Heart Blood cells HEK Platelet |
Volani 2016 Abstract Mito Xmas Meeting Innsbruck | 2016 | Effects of systemic iron perturbations on mitochondrial activity and on cellular metabolism in vivo. | Mouse | Liver |
Sumbalova 2015 Abstract MiP2015 | 2015 | High-resolution measurement of mitochondrial membrane potential and respiration â comparison of potentiometric and fluorometric methods. | Mouse | Nervous system |
Laner 2015 Abstract MiPschool Greenville 2015 | 2015 | Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes. | Dog | Skeletal muscle |
Sumbalova 2014 Abstract MiP2014 | 2014 | Optimization of malate concentration for high-resolution respirometry: mitochondria from rat liver and brain. | Rat | Nervous system Liver |