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| == O2k signals ==
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| Three electronic '''channel types''' are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A (left) and B (right), based on signals (numerical) sent at a fixed data sampling time interval (typical: 1 s or 2 s; range 0.2 s to >10 s):
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| [[Image:O2k-Core plugs-for-modules.jpg|right|300px|O2k-Core.jpg]]
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| : '''1. O<sub>2</sub> channel''' with electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The [[O2k-Core]] supports not only the O<sub>2</sub> channel (O2 plug), but also the channel types 2 and 3 for the add-on modules.
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| : '''2. Amp channel''': The amperometric channel of the O2k (Amp plug), has electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp channel is used with the [[O2k-Fluo LED2-Module]] and the [[O2k-NO Amp-Module]].
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| : '''3. pX channel''' with electric potential (volt [V]) as the primary and raw signal (BNC pX plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref).
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| '''NextGen-O2k''': additional channel types are available in the [[NextGen-O2k]]:
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| : '''4. Dual-fluo channel'''.
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| : '''5. Spectrophotometric channel'''.
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| '''System channels''', for instrumental control, not separated into O2k-Chamber A and B (single-data), based on signals (numerical) sent at the same fixed data sampling time interval:
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| : 6a. Block temperature: Measured block temperature equivalent to the experimental temperature in the O2k-Chambers at steady state [°C].
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| : 6b. Peltier power (system channel): Relative heating or cooling power of the Peltier system.
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| : 6c. Barometric pressure (system channel): Measured barometric pressure [kPa].
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| : 6d. Ambient temperature (system channel): Measured temperature of room air (O2k-Series G upwards) [°C].
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| : 6e. External temperature (system channel): Temperature measured with an independently placed thermistor (O2k-Series G upwards) [°C].
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| '''Events''' carry information on a time point during the experiment, a short event name (text), and a longer string of comments (text):
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| : 7. Events: Automatic events (e.g. from the [[TIP2k]]) and manually triggered events.
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| == O2k output ==
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| The 'output' of a channel is the finally important information, which may be summarized in a data base.
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| '''Calibrated signals and derived plots''': Three types of output can be derived from the signal of the channels supported by the O2k-Core and displayed as separate plots:
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| : '''I: Concentration'''. Examples: O<sub>2</sub>; Ca<sup>2+</sup>; [[concentration]] is amount of substance per volume [1 µM = 1 µmol·dm<sup>-3</sup> = 1 nmol·ml<sup>-1</sup>].
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| : '''II: Flow, flux, rate'''. Examples: O<sub>2</sub> consumption; H<sub>2</sub>O<sub>2</sub> production; volume-specific [[flux]] is the negative or positive time derivative of concentration over time, frequently multiplied by 1,000 [pmol·s<sup>-1</sup>·ml<sup>-1</sup>]. Volume-specific flux normalized for the cell density [10<sup>6</sup>·ml<sup>-1</sup>] is [[flow]] [pmol·s<sup>-1</sup>·ml<sup>-1</sup>]. Volume-specific flux normalized for the mass density of the sample in the O2k-Chamber [mg·ml<sup>-1</sup>] is mass-specific flux [pmol·s<sup>-1</sup>·mg<sup>-1</sup>]. Flux in any respiratory state normalized for flux in a defined reference state is a rate [dimensionless] or [[flux control ratio]].
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| : '''III: Force, potential'''. Examples: [[TMRM]] and [[Safranin]] for mt-membrane potential; redox potential from redox states of cytochromes or NADH .
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| [[Image:Oxygraph-2k-Concept.jpg|right|300px|O2k-Concept.jpg]]
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| '''Output types for the O2k-MultiSensor modules'''
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| : 1. O<sub>2</sub> channel - [[O2k-Core]]: Output type I and II.
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| : 2a. Amp channel - [[O2k-Fluo LED2-Module]]: The focus of the output depends on the specific fluorophore. Output type I, II, or III, or any combination may relate to the primary level of interest.
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| : 2b. Amp channel - [[O2k-NO Amp-Module]]: Output type I and II.
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| : 3a. pX channel - [[O2k-TPP+ ISE-Module]]: Output type III.
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| : 3b. pX channel - [[O2k-pH ISE-Module]]: Output type I and II.
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| '''Additional output types for the NextGen-O2k'''
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| : 4. Single-fluo channel - [[NextGen-O2k]]: Plots of one selected wavelength can be selected for recording. Output type I(1), II(1), III(1) depends on the fluorophore.
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| : 5. Dual-fluo channel - [[NextGen-O2k]]: Plots of two selected wavelengths can be selected for simultaneous recording. Output type I(2), II(2), III(2) depends on the fluorophore.
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| : 6. Spectrophotometric channel - [[NextGen-O2k]]: Output type IV (three plots for redox states of cytochromes ''aa''<sub>3</sub>, ''b'', and ''c'').
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| : '''V''': Pre-calibrated signals for system channels.
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| : '''VI''': Events.
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