Octanoylcarnitine: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Oct | |abbr=Oct | ||
|description=''' | |description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]]. | ||
|info=[http://www.jbc.org/content/256/16/8371.full.pdf ''pdf''] | |info=[http://www.jbc.org/content/256/16/8371.full.pdf ''pdf''] | ||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | |||
{{MitoPedia methods|type=Substrate ETS | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
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|type=Substrate ETS | |type=Substrate ETS | ||
}} | }} | ||
'''Carnitine O-octanoyltransferase''' is an enzyme that catalyzes the chemical reaction: | '''Carnitine O-octanoyltransferase''' is an enzyme that catalyzes the chemical reaction: | ||
::''octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine'' | ::''octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine'' | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Oct: | '''Oct: Octanoylcarnitine''' (DL-Octanoyl-carnitine-HCL, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85. | ||
'''Alternative:''' (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86. | '''Alternative:''' (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86. | ||
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'''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
:: | ::# Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H<sub>2</sub>O. | ||
:: | ::# Divide into 0.2 ml portions. | ||
:: | ::# Store at -20 °C. | ||
'''Oxygraph-2k | '''Oxygraph-2k manual titrations into 2 ml O2k-chamber''' | ||
::* Titration volume: 4 µl using a 10 µl syringe | ::* Titration volume: 4 µl using a 10 µl syringe. | ||
::* Final | ::* Final concentration: 0.2 mM in MiR05 or [[MiR06]]. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06. | ||
Revision as of 09:34, 3 September 2014
- high-resolution terminology - matching measurements at high-resolution
Octanoylcarnitine
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine.
Abbreviation: Oct
Reference: pdf
MitoPedia topics: Substrate and metabolite
Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:
- octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine
Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.
Application in HRR
Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 100 mM stock solution (dissolved in H2O):
- Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H2O.
- Divide into 0.2 ml portions.
- Store at -20 °C.
Oxygraph-2k manual titrations into 2 ml O2k-chamber
- Titration volume: 4 µl using a 10 µl syringe.
- Final concentration: 0.2 mM in MiR05 or MiR06. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06.
