Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "OroboPOS"

From Bioblast
Β 
(14 intermediate revisions by 4 users not shown)
Line 1: Line 1:
{{Product
{{Technical support}}
|description=The '''OroboPOS''' is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.
__TOC__
{{MitoPedia without banner
|abbr=
|description=[[Image:OroboPOS.JPG|right|180px]]
The '''OroboPOS''' is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.


It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 Β΅m membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.
It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 Β΅m membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.
|product id=26000-01
|info=[https://www.oroboros.at/index.php/product/orobopos/ '''Product details and purchase information''']
|product type=O2k, OroboPOS
Β 
|info=[[MiPNet19.18B POS-service]], [[Gnaiger 1983 POS]]
|product image=[[Image:OroboPOS sensor head.jpg|right|180px]]
}}
}}
== Additional resources ==
:::Β» [[MiPNet19.18B POS-service]]
:::Β» [[Gnaiger 1983 POS]]Β 
:::Β» [[Polarographic_oxygen_sensor]]
{{MitoPedia O2k and high-resolution respirometry
{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=O2k hardware
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
}}
}}
== Specifications ==
'''From nM to mM O<sub>2</sub> - a million-fold dynamic range''': The dynamic range of oxygen measurement spans from air saturation (about 200 Β΅M) to the normoxic intracellular range (10-30 Β΅M) and severe hypoxia (<0.1 Β΅M), but also to hyperoxia (oxygen saturation: c. 1 mM). In measurements of cell respiration in conjunction with the [[Titration-Injection microPump|Titration-Injection microPump TIP2k]], allow accurate steady-state measurement of oxgen levels and respiration with resolution of better than Β±1 nM O<sub>2</sub>.
== Shipment ==
* [[O2k-Core]]: [[3 OroboPOS]] are included and shipped in the [[O2k-Service Box]]. The spare (third) OroboPOS is added to the items ordered with the O2k-System.
OroboPOS are protected in a transparent perspex vial screwed onto a blue POM base, with the [[OroboPOS-Membrane Ring]] arrested on the POS shaft between the POM base and the thicker part of the POS head which holds an [[O-ring\Viton\8x1 mm]].
== POS service and membrane mounting ==
:>> [[MiPNet19.18B POS-service |O2k-Manual for OroboPOS service]]
:>> [[OroboPOS-Service Kit]]
== POS calibration and SOP ==
:>> [[MiPNet19.18D O2k-calibration]]
:>> [[MiPNet06.03 POS-calibration-SOP]]
== References ==
* Gnaiger E, Forstner H, eds (1983) Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Springer, Berlin, Heidelberg, New York:370 pp.
* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97.
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52.
[[Category:Technical service]]

Latest revision as of 16:41, 10 November 2020


                  


O2k-Open Support

OroboPOS



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

OroboPOS.JPG

The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.

It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 Β΅m membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.


Reference: Product details and purchase information

Additional resources

Β» MiPNet19.18B POS-service
Β» Gnaiger 1983 POS
Β» Polarographic_oxygen_sensor

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry