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| |description=The '''OroboPOS''' is a polarographic oxygen sensor (POS), with an amperometric mode of operation. With application of a polarization voltage (0.6 to 0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage. | | __TOC__ |
| | {{MitoPedia without banner |
| | |abbr= |
| | |description=[[Image:OroboPOS.JPG|right|180px]] |
| | The '''OroboPOS''' is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range. |
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| | It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage. |
| | |info=[https://www.oroboros.at/index.php/product/orobopos/ '''Product details and purchase information'''] |
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| Shipment: OroboPOS are protected in a transparent perspex vial screwed onto a blue POM base, with the [[OroboPOS-Membrane Ring]] arrested on the POS shaft between the POM base and the thicker part of the POS head which holds an [[O-ring\Viton\8x1 mm]].
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| |product id=26000-01
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| |product type=O2k, OroboPOS
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| |info=Previous Product ID 26100-24, [[MiPNet08.04]], [[Gnaiger_1983_POS]]
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| |product image=[[File:OroboPOS sensor head.jpg|right|180px|link=]]
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| }} | | }} |
| * [[O2k-Core]]: [[2 OroboPOS]] are included. A spare (third) OROBoPOS may be added to the items ordered with the O2k-System. OroboPOS are shipped with the O2k in the [[O2k-Service Box]].
| | == Additional resources == |
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| | | :::» [[MiPNet19.18B POS-service]] |
| === POS Service and Membrane Mounting ===
| | :::» [[Gnaiger 1983 POS]] |
| Please refer to the newest version of the manual [[B MiPNet08.04 POS-Service]]. If a [[Stirrer test]] shows a slow response of the sensor some hints in [[Slow responding sensor]].
| | :::» [[Polarographic_oxygen_sensor]] |
| | | {{MitoPedia O2k and high-resolution respirometry |
| == Trouble shooting ==
| | |mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry |
| | | }} |
| As the heart of the OROBOROS Oxygraph the [[Polarographic oxygen sensor]] is of course the prime suspect when any kind of troubles are encountered. However, frequently problems can be traced either to inadequate software settings or to other hardware components (like the [[POS connector (technical service) |POS connector]]).
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| Therefore it is important to confirm the problem doing a [[Sensor test]] and to [[Locating a problem |localize the problem]].
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| ===Problems that require a full Sensor test and localization of the problem===
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| *unstable O2 signal
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| *unstable O2 flux
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| *high O2 signal at air saturation
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| *high O2 signal at zero oxygen
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| ===Special cases: no further localization necessary ===
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| * [[Slow responding sensor]], if this has been confirmed by a [[Stirrer test]]. Note: The stirrer test is part of the sensor test. It is therefore recommended to do a full [[Sensor test]] whenever problems are encountered.
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| ===General Trouble Shooting Procedure for the OroPos ===
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| Confirm the problem by doing a [[Sensor test]] in the absence of biological material
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| ====Option 1.A: No problem was visible in the [[Sensor test]]====
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| check the following DatLab settings
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| * [[Gain (O2 channel)]]
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| * [[POS calibration]]
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| * Scaling
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| If the problem is not visible when observing the [[Raw signal]] but is visible when observing the calibrated oxygen signal, then there is probable a problem with the [[POS calibration]].
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| ====Option 1.B: The problem was visible in the [[Sensor test]]====
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| * proceed to [[ Locating a problem|Locate the Problem ]] and the continue as stated below
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| ====Option 2.A: The problem could be localized on the [[POS]]====
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| '''Solutions 2.A'''
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| Follow the instructions for oxygen sensor service [http://www.oroboros.at/index.php?id=o2k-pos-service [MiPNet08.04]]. Use this opportunity to apply [[contact oil]] to the gold pin and thread connecting [[POS]] and [[sensor connector]]. After a sensor service or after applying anew membrane allow the oxygraph to run over night to achieve a stable signal before repeating the [[sensor test]].
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| If all service precautions fail to solve the problem, the [[POS]] has to be sent to us for further service (a recovery can not be guaranteed), or a new sensor is to be applied.
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| ====Option 2.B: The problem was not localized on the [[POS]]====
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| '''Solutions 2.B'''
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| If the problem was localized on an other component follow the instructions for this component. If you could not locate the problem contact OROBOROS Instruments.
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| === OrboPOS Technical Service Pages ===
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| {{#ask: mainlabel=Title|[[Category:All]] | [[Technical service::O2 sensor Technical service::O2 signal]]| ?Technical service}} | |
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| {{#set: Technical service=O2 sensor|Technical service=O2 signal}}
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| {{Technical service}}
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| __SHOWFACTBOX__
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MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.
It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.
Reference: Product details and purchase information
Additional resources
- » MiPNet19.18B POS-service
- » Gnaiger 1983 POS
- » Polarographic_oxygen_sensor
MitoPedia O2k and high-resolution respirometry:
O2k hardware,
O2k-Open Support,
O2k-Respirometry,
O2k-FluoRespirometry
